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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNA ligase III/LIG3 Monoclonal antibody (68810-1-Ig)

    WB analysis using 68810-1-Ig (same clone as 68810-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68810-1-Ig (DNA ligase III/LIG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68810-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using DNA ligase III/LIG3 Monoclonal antibody (68810-1-Ig)

    WB analysis of HSC-T6 using 68810-1-Ig (same clone as 68810-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68810-1-Ig (LIG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68810-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50683-2

    Cytometric bead array standard curve of MP50683-2

    Cytometric bead array standard curve of MP50683-2, DNA ligase III/LIG3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68810-1-PBS. Detection antibody: 68810-3-PBS. Standard:Ag31484. Range: 1.563-100 ng/mL.

  • Cytometric bead array standard curve of MP50683-3

    Cytometric bead array standard curve of MP50683-3

    Cytometric bead array standard curve of MP50683-3, DNA ligase III/LIG3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68810-1-PBS. Detection antibody: 68810-2-PBS. Standard:Ag31484. Range: 1.563-100 ng/mL.

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DNA ligase III/LIG3 Monoclonal antibody, PBS Only (Capture)

DNA ligase III/LIG3 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 68810-1-PBS
Clone No.1B10B3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

LIG3, LIG2, EC:6.5.1.1, DNA ligase III, DNA ligase 3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68810-1-PBS targets DNA ligase III/LIG3 as part of a matched antibody pair:

MP50683-2: 68810-1-PBS capture and 68810-3-PBS detection (validated in Cytometric bead array)

MP50683-3: 68810-1-PBS capture and 68810-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DNA ligase III/LIG3 fusion protein Ag31484 相同性解析による交差性が予測される生物種
Full Name ligase III, DNA, ATP-dependent
Calculated molecular weight 100-110 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC068005
Gene Symbol LIG3
Gene ID (NCBI) 3980
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP49916
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

DNA ligase III (DNA ligase 3) is an enzyme that in humans is encoded by the LIG3 gene. The human LIG3 gene encodes ATP-dependent DNA ligases that seal interruptions in the phosphodiester backbone of duplex DNA. There are three families of ATP-dependent DNA ligases in eukaryotes. These enzymes utilize the same three step reaction mechanism; 1 formation of a covalent enzyme-adenylate intermediate; 2 transfer of the adenylate group to the 5' phosphate terminus of a DNA nick; 3 phosphodiester bond formation. Unlike LIG1 and LIG4 family members that are found in almost all eukaryotes, LIG3 family members are less widely distributed. The LIG3 gene encodes several distinct DNA ligase species by alternative translation initiation and alternative splicing mechanisms.