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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LIG3 Monoclonal antibody, PBS Only (Capture)

LIG3 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B10B3

Cat no : 68810-1-PBS

Synonyms

ligase III, DNA, ATP dependent, LIG2, EC:6.5.1.1, DNA ligase III, DNA ligase 3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using LIG3 Monoclonal antibody (68810-1-Ig)

    WB analysis using 68810-1-Ig (same clone as 68810-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68810-1-Ig (LIG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68810-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using LIG3 Monoclonal antibody (68810-1-Ig)

    WB analysis of HSC-T6 using 68810-1-Ig (same clone as 68810-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68810-1-Ig (LIG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68810-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50683-2

    Cytometric bead array standard curve of MP50683-2

    Cytometric bead array standard curve of MP50683-2, LIG3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68810-1-PBS. Detection antibody: 68810-3-PBS. Standard:Ag31484. Range: 1.563-100 ng/mL.

  • Cytometric bead array standard curve of MP50683-3

    Cytometric bead array standard curve of MP50683-3

    Cytometric bead array standard curve of MP50683-3, LIG3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68810-1-PBS. Detection antibody: 68810-2-PBS. Standard:Ag31484. Range: 1.563-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68810-1-PBS targets LIG3 as part of a matched antibody pair:

MP50683-2: 68810-1-PBS capture and 68810-3-PBS detection (validated in Cytometric bead array)

MP50683-3: 68810-1-PBS capture and 68810-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LIG3 fusion protein Ag31484 相同性解析による交差性が予測される生物種
Full Name ligase III, DNA, ATP-dependent
Calculated molecular weight 100-110 kDa
GenBank accession numberBC068005
Gene symbol LIG3
Gene ID (NCBI) 3980
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.