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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using EPLIN Monoclonal antibody (66071-1-Ig)

    WB analysis of HEK-293 using 66071-1-Ig

    WB result of EPLIN antibody (66071-1-Ig, 1:2000) with si-Control and si-EPLIN transfected HEK293 cells.

  • Western Blot (WB) analysis of various lysates using EPLIN Monoclonal antibody (66071-1-Ig)

    WB analysis using 66071-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using EPLIN Monoclonal antibody (66071-1-Ig)

    WB analysis using 66071-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using EPLIN Monoclonal antibody (66071-1-Ig)

    WB analysis of HeLa using 66071-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using EPLIN Monoclonal antibody (66071-1-Ig)

    WB analysis of human brain using 66071-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using EPLIN Monoclonal antibody (66071-1-Ig)

    WB analysis of HEK-293 using 66071-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using EPLIN Monoclonal antibody (66071-1-Ig)

    WB analysis of COLO 320 using 66071-1-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using EPLIN Monoclonal antibody (66071-1-Ig)

    IHC staining of human heart using 66071-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart using 66071-1-Ig(EPLIN antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using EPLIN Monoclonal antibody (66071-1-Ig)

    IHC staining of human heart using 66071-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart using 66071-1-Ig(EPLIN antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EPLIN Monoclonal antibody (66071-1-Ig)

    IF Staining of HeLa using 66071-1-Ig

    Immunofluorescent analysis of Hela cells using 66071-1-Ig (EPLIN antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG.

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  • Featured Product
  • KD/KO Validated

EPLIN Monoclonal antibody

EPLIN Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66071-1-Ig
Clone No.6E6E6
Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Synonyms

LIMA1, SREBP3, PP624, LIM domain and actin-binding protein 1, LIM domain and actin binding 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, A431 cells, COLO 320 cells, HEK-293 cells, human brain tissue, HaCaT cells, SCaBER cells, MDA-MB-231 cells, SK-BR-3 cells, HT-29 cells, pig colon tissue, rat colon tissue, HSC-T6 cells, ROS1728 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66071-1-Ig targets EPLIN in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EPLIN fusion protein Ag13288 相同性解析による交差性が予測される生物種
Full Name LIM domain and actin binding 1
Calculated molecular weight 85 kDa
Observed molecular weight 90 kDa, 110 kDa
GenBank accession numberBC001247
Gene Symbol EPLIN
Gene ID (NCBI) 51474
RRIDAB_11043341
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UHB6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EPLIN is a cytoskeletal protein that is preferentially expressed in epithelial cells and is implicated in regulation of cytoskeletal dynamics and cytokinesis. In human, there are two known isoforms, EPLIN-a and -b, generated by alternative promoter usage from a single gene. EPLIN-a expression is often down-regulated in cancerous cells and tissues. This antibody recognizes both of isoforms of EPLN.

Protocols

Product Specific Protocols
WB protocol for EPLIN antibody 66071-1-IgDownload protocol
IHC protocol for EPLIN antibody 66071-1-IgDownload protocol
IF protocol for EPLIN antibody 66071-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanWB,IHC,IF,CoIP

Cell Mol Biol Lett

Lipotoxic hepatocyte derived LIMA1 enriched small extracellular vesicles promote hepatic stellate cells activation via inhibiting mitophagy

Authors - Shihui Li
  • KD Validated
View Article