• Featured Product
  • KD/KO Validated

EPLIN Monoclonal antibody

EPLIN Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

6E6E6

Cat no : 66071-1-Ig

Synonyms

LIMA1, SREBP3, PP624, LIM domain and actin-binding protein 1, LIM domain and actin binding 1


☆対象製品が30%オフ!組換えタンパク質&免染用試薬 >>> キャンペーン実施中!


Tested Applications

Positive WB detected inHeLa cells, A431 cells, COLO 320 cells, HEK-293 cells, human brain tissue, HaCaT cells, SCaBER cells, MDA-MB-231 cells, SK-BR-3 cells, HT-29 cells, pig colon tissue, rat colon tissue, HSC-T6 cells, ROS1728 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66071-1-Ig targets EPLIN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EPLIN fusion protein Ag13288 相同性解析による交差性が予測される生物種
Full Name LIM domain and actin binding 1
Calculated molecular weight 85 kDa
Observed molecular weight 90 kDa, 110 kDa
GenBank accession numberBC001247
Gene symbol EPLIN
Gene ID (NCBI) 51474
RRIDAB_11043341
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EPLIN is a cytoskeletal protein that is preferentially expressed in epithelial cells and is implicated in regulation of cytoskeletal dynamics and cytokinesis. In human, there are two known isoforms, EPLIN-a and -b, generated by alternative promoter usage from a single gene. EPLIN-a expression is often down-regulated in cancerous cells and tissues. This antibody recognizes both of isoforms of EPLN.

Protocols

Product Specific Protocols
WB protocol for EPLIN antibody 66071-1-IgDownload protocol
IHC protocol for EPLIN antibody 66071-1-IgDownload protocol
IF protocol for EPLIN antibody 66071-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols