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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated EPLIN Monoclonal antibody

EPLIN Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

6E6E6

Cat no : CL488-66071

Synonyms

EPLIN, FLJ38853, LIM domain and actin binding 1, LIMA1, SREBP3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated EPLIN Monoclonal ant (CL488-66071)

    IF Staining of HeLa using CL488-66071

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL488-66071 (EPLIN antibody) at dilution of 1:100.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-66071 is EPLIN Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EPLIN fusion protein Ag13288 相同性解析による交差性が予測される生物種
Full Name LIM domain and actin binding 1
Calculated molecular weight 85 kDa
Observed molecular weight 90 kDa, 110 kDa
GenBank accession numberBC001247
Gene symbol EPLIN
Gene ID (NCBI) 51474
RRIDAB_2883250
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EPLIN is a cytoskeletal protein that is preferentially expressed in epithelial cells and is implicated in regulation of cytoskeletal dynamics and cytokinesis. In human, there are two known isoforms, EPLIN-a and -b, generated by alternative promoter usage from a single gene. EPLIN-a expression is often down-regulated in cancerous cells and tissues. This antibody recognizes both of isoforms of EPLN.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 EPLIN antibody CL488-66071Download protocol
Standard Protocols
Click here to view our Standard Protocols