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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LIMK2 Recombinant antibody, PBS Only

LIMK2 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230283D10

Cat no : 82977-1-PBS

Synonyms

LIMK-2, LIMK 2, LIM domain kinase 2, EC:2.7.11.1, 230283D10

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LIMK2 Recombinant antibody (82977-1-RR)

    IF Staining of HepG2 using 82977-1-RR (same clone as 82977-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LIMK2 antibody (82977-1-RR, Clone: 230283D10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 82977-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using LIMK2 Recombinant antibody (82977-1-RR)

    FC experiment of HepG2 using 82977-1-RR (same clone as 82977-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug LIMK2 Recombinant antibody (82977-1-RR, Clone:230283D10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82977-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82977-1-RR

    Affinity and Kinetic Characterization of 82977-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82977-1-RR against Human LIMK2 were performed. The affinity constant is 4.03 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82977-1-PBS targets LIMK2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen LIMK2 fusion protein Ag3011 相同性解析による交差性が予測される生物種
Full Name LIM domain kinase 2
Calculated molecular weight 686 aa, 78 kDa
GenBank accession numberBC013051
Gene symbol LIMK2
Gene ID (NCBI) 3985
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.