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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated LIN28A Monoclonal antibody

LIN28A Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4F5G6

Cat no : CL488-60344

Synonyms

LIN28, Lin-28A, Lin 28A, LIN 28, CSDD1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human prostate cancer tissue using CoraLite® Plus 488-conjugated LIN28A Monoclonal an (CL488-60344)

    IF Staining of human prostate cancer using CL488-60344

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CoraLite® Plus 488 LIN28 antibody (CL488-60344, Clone: 4F5G6 ) at dilution of 1:200.

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Tested Applications

Positive IF-P detected inhuman prostate cancer tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60344 targets LIN28A in IF-P applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LIN28A fusion protein Ag2312 相同性解析による交差性が予測される生物種
Full Name lin-28 homolog (C. elegans)
Calculated molecular weight 209 aa, 23 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC028566
Gene symbol LIN28
Gene ID (NCBI) 79727
RRIDAB_2919251
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LIN28 is one of the four key human factors (OCT4, SOX2, NANOG and LIN28) used to reprogram human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (Ips) cell. Lin28 is a marker of undifferentiated human embryonic stem cells and a cytoplasmic Mrna-binding protein that binds to and enhances the translation of the IGF2 Mrna. LIN28 has also been shown to bind to the let-7 pre-miRNA and block production of the mature let-7 microRNA in mouse embryonic stem cells. The calculated molecular weight of LIN28 is 23 kDa, but the modified LIN28 is about 28 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 LIN28A antibody CL488-60344Download protocol
Standard Protocols
Click here to view our Standard Protocols