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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LINGO1 Recombinant antibody (84375-3-RR)

    WB analysis using 84375-3-RR (same clone as 84375-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84375-3-RR (LINGO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84375-3-PBS in a different storage buffer formulation.

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LINGO1 Recombinant antibody, PBS Only

LINGO1 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 84375-3-PBS
Clone No.241599A9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Leucine-rich repeat neuronal protein 6A, Leucine-rich repeat neuronal protein 1, Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1, Leucine-rich repeat and immunoglobulin domain-containing protein 1, LERN1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84375-3-PBS targets LINGO1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen LINGO1 fusion protein Ag13597 相同性解析による交差性が予測される生物種
Full Name leucine rich repeat and Ig domain containing 1
Calculated molecular weight 70 kDa
Observed molecular weight83 kDa
GenBank accession numberBC011057
Gene Symbol LINGO1
Gene ID (NCBI) 84894
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96FE5
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

LINGO-1 is a transmembrane receptor that contains 12 leucine-rich repeats and an immunoglobulin domain. It is expressed primarily in the central nervous system. LINGO-1 binds Nogo-66 receptor (NgR) and p75 and that is a functional component of the NgR/p75 signaling complex (PMID: 14966521). This protein has a calculated molecular weight of 70 kDa and migrates at higher molecular weight due to glycosylation (PMID: 21872576; 25257685; 18326721).