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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LINGO1 Recombinant antibody

LINGO1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241599A9

Cat no : 84375-3-RR

Synonyms

Leucine-rich repeat neuronal protein 6A, Leucine-rich repeat neuronal protein 1, Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1, Leucine-rich repeat and immunoglobulin domain-containing protein 1, LERN1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LINGO1 Recombinant antibody (84375-3-RR)

    WB analysis using 84375-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84375-3-RR (LINGO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue, fetal human brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84375-3-RR is LINGO1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen LINGO1 fusion protein Ag13597 相同性解析による交差性が予測される生物種
Full Name leucine rich repeat and Ig domain containing 1
Calculated molecular weight 70 kDa
Observed molecular weight83 kDa
GenBank accession numberBC011057
Gene symbol LINGO1
Gene ID (NCBI) 84894
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LINGO-1 is a transmembrane receptor that contains 12 leucine-rich repeats and an immunoglobulin domain. It is expressed primarily in the central nervous system. LINGO-1 binds Nogo-66 receptor (NgR) and p75 and that is a functional component of the NgR/p75 signaling complex (PMID: 14966521). This protein has a calculated molecular weight of 70 kDa and migrates at higher molecular weight due to glycosylation (PMID: 21872576; 25257685; 18326721).

Protocols

Product Specific Protocols
WB protocol for LINGO1 antibody 84375-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols