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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Lamin B2 Recombinant antibody (82801-3-RR)

    IF Staining of HepG2 using 82801-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Lamin B2 antibody (82801-3-RR, Clone: 230184G4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using Lamin B2 Recombinant antibody (82801-3-RR)

    FC experiment of HepG2 using 82801-3-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Lamin B2 Recombinant antibody (82801-3-RR, Clone:230184G4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 82801-3-RR

    Affinity and Kinetic Characterization of 82801-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82801-3-RR against Human Lamin B2 were performed. The affinity constant is 0.428 nM.

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Lamin B2 Recombinant antibody

Lamin B2 Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 82801-3-RR
Clone No.230184G4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

LMNB2, LMN2, Lamin-B2, LAMB2, 230184G4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82801-3-RR targets Lamin B2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Lamin B2 fusion protein Ag1335 相同性解析による交差性が予測される生物種
Full Name lamin B2
Calculated molecular weight 68 kDa
GenBank accession numberBC006551
Gene Symbol Lamin B2
Gene ID (NCBI) 84823
RRIDAB_3670554
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ03252
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Lamins are nuclear membrane structural components that are important in structural integrity of the nucleus and may also interact with chromatin (PMID: 33033404). Research studies show that lamin B2 knockout mice exhibit neuronal developmental defects and that both proteins are essential for typical brain development (PMID: 20145110). Mutations in Lamin B2 can result in a susceptibility to developing acquired partial lipodystrophy, a rare disorder characterized by the progressive loss of subcutaneous fat in a bilaterally symmetrical fashion (PMID: 16826530).

Protocols

Product Specific Protocols
IF protocol for Lamin B2 antibody 82801-3-RRDownload protocol
FC protocol for Lamin B2 antibody 82801-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols