• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LMOD1 Monoclonal antibody

LMOD1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D6H2

Cat no : 60414-1-Ig

Synonyms

Thyroid-associated ophthalmopathy autoantigen, SM-Lmod, Leiomodin-1, Leiomodin1, 1D6H2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using LMOD1 Monoclonal antibody (60414-1-Ig)

    WB analysis using 60414-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60414-1-Ig (LMOD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using LMOD1 Monoclonal antibody (60414-1-Ig)

    WB analysis using 60414-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60414-1-Ig (LMOD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using LMOD1 Monoclonal antibody (60414-1-Ig)

    IHC staining of human colon using 60414-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60414-1-Ig (LMOD1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive WB detected inpig colon tissue, ARPE-19 cells, hTERT-RPE1 cells, rabbit colon tissue, rat colon tissue, mouse colon tissue, rabbit testis tissue, rat uterus tissue, mouse uterus tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60414-1-Ig targets LMOD1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LMOD1 fusion protein Ag7568 相同性解析による交差性が予測される生物種
Full Name leiomodin 1 (smooth muscle)
Calculated molecular weight 67 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC001755
Gene symbol LMOD1
Gene ID (NCBI) 25802
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LMOD1 was initially identified as a 64-kDa autoantigen in an expression screen with sera from patients with Hashimoto's thyroiditis. Recently immunohistochemistry and immunoblotting of LMOD1 demonstrated its highly restrictive pattern of expression in SMC lineages,like adult aorta, and bladder. LMOD1 is also shown to exhibit an embryonic and adult expression pattern. These data identify LMOD1 as a new SMC-restricted gene associated with the SMC differentiated phenotype.

Protocols

Product Specific Protocols
WB protocol for LMOD1 antibody 60414-1-IgDownload protocol
IHC protocol for LMOD1 antibody 60414-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols