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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LMOD1 Monoclonal antibody, PBS Only

LMOD1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D6H2

Cat no : 60414-1-PBS

Synonyms

Thyroid-associated ophthalmopathy autoantigen, SM-Lmod, Leiomodin-1, Leiomodin1, 1D6H2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using LMOD1 Monoclonal antibody (60414-1-Ig)

    WB analysis using 60414-1-Ig (same clone as 60414-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60414-1-Ig (LMOD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60414-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using LMOD1 Monoclonal antibody (60414-1-Ig)

    WB analysis using 60414-1-Ig (same clone as 60414-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60414-1-Ig (LMOD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60414-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using LMOD1 Monoclonal antibody (60414-1-Ig)

    IHC staining of human colon using 60414-1-Ig (same clone as 60414-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60414-1-Ig (LMOD1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60414-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60414-1-PBS targets LMOD1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LMOD1 fusion protein Ag7568 相同性解析による交差性が予測される生物種
Full Name leiomodin 1 (smooth muscle)
Calculated molecular weight 67 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC001755
Gene symbol LMOD1
Gene ID (NCBI) 25802
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

LMOD1 was initially identified as a 64-kDa autoantigen in an expression screen with sera from patients with Hashimoto's thyroiditis. Recently immunohistochemistry and immunoblotting of LMOD1 demonstrated its highly restrictive pattern of expression in SMC lineages,like adult aorta, and bladder. LMOD1 is also shown to exhibit an embryonic and adult expression pattern. These data identify LMOD1 as a new SMC-restricted gene associated with the SMC differentiated phenotype.