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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using LMX1B Polyclonal antibody (18278-1-AP)

    WB analysis of HeLa using 18278-1-AP (same clone as 18278-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 18278-1-AP (LMX1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18278-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of embryonic kidney tissue using LMX1B Polyclonal antibody (18278-1-AP)

    IF Staining of embryonic kidney tissue using 18278-1-AP (same clone as 18278-1-PBS)

    IF result of anti-LMX1B (18278-1-AP ,1:900) with embryonic kidney by Dr. Joo-Seop Park. Red: Podocytes, anti-rabbir Alexa555; Blue: Hoechst. This data was developed using the same antibody clone with 18278-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

LMX1B Polyclonal antibody, PBS Only

LMX1B Polyclonal Antibody for WB, IF, Indirect ELISA
Cat No. 18278-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18278-1-PBS targets LMX1B in WB, IF, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LMX1B fusion protein Ag13096 相同性解析による交差性が予測される生物種
Full Name LIM homeobox transcription factor 1, beta
Calculated molecular weight 372 aa, 42 kDa
Observed molecular weight 42-45 kDa
GenBank accession numberBC112120
Gene Symbol LMX1B
Gene ID (NCBI) 4010
RRIDAB_11232226
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60663
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

LMX1B, LIM homeodomain factor 1b, functions as a transcription factor, and is essential for the normal development of dorsal limb structures, the glomerular basement membrane, the anterior segment of the eye, and dopaminergic and serotonergic neurons. Lmx1b is a continuously expressed, terminal selector-type factor in 5-HT neurons. It is required for maintenance of central serotonergic neurons (PMID: 17151281, PMID: 14602809). It is expressed in brain, testis, skeletal muscle, and pancreatic islets (PMID: 35417751, PMID: 18512225).