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Host
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LONP1 Recombinant antibody, PBS Only (Capture)

LONP1 Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240460E5

Cat no : 83552-3-PBS

Synonyms

Mitochondrial ATP-dependent protease Lon, LONP 1, LONP, LON, EC:3.4.21.53

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LONP1 Recombinant antibody (83552-3-RR)

    IF Staining of HepG2 using 83552-3-RR (same clone as 83552-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LONP1 antibody (83552-3-RR, Clone: 240460E5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83552-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LONP1 Recombinant antibody (83552-3-RR)

    IF Staining of HepG2 using 83552-3-RR (same clone as 83552-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LONP1 antibody (83552-3-RR, Clone: 240460E5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83552-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using LONP1 Recombinant antibody (83552-3-RR)

    FC experiment of HeLa using 83552-3-RR (same clone as 83552-3-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug LONP1 Recombinant antibody (83552-3-RR, Clone:240460E5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83552-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00531-3

    Cytometric bead array standard curve of MP00531-3

    Cytometric bead array standard curve of MP00531-3, LONP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83552-3-PBS. Detection antibody: 83552-1-PBS. Standard: Ag7196. Range: 0.938-60 ng/mL.

  • Affinity and Kinetic Characterization of 83552-3-RR

    Affinity and Kinetic Characterization of 83552-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83552-3-RR against Human LONP1 were performed. The affinity constant is 1.38 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83552-3-PBS targets LONP1 as part of a matched antibody pair:

MP00531-3: 83552-3-PBS capture and 83552-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen LONP1 fusion protein Ag7196 相同性解析による交差性が予測される生物種
Full Name lon peptidase 1, mitochondrial
Calculated molecular weight 106 kDa
GenBank accession numberBC000235
Gene symbol LONP1
Gene ID (NCBI) 9361
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.