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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LONP1 Recombinant antibody

LONP1 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240460E5

Cat no : 83552-3-RR

Synonyms

Mitochondrial ATP-dependent protease Lon, LONP 1, LONP, LON, EC:3.4.21.53

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LONP1 Recombinant antibody (83552-3-RR)

    IF Staining of HepG2 using 83552-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LONP1 antibody (83552-3-RR, Clone: 240460E5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LONP1 Recombinant antibody (83552-3-RR)

    IF Staining of HepG2 using 83552-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LONP1 antibody (83552-3-RR, Clone: 240460E5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using LONP1 Recombinant antibody (83552-3-RR)

    FC experiment of HeLa using 83552-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug LONP1 Recombinant antibody (83552-3-RR, Clone:240460E5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83552-3-RR

    Affinity and Kinetic Characterization of 83552-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83552-3-RR against Human LONP1 were performed. The affinity constant is 1.38 nM.

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83552-3-RR is LONP1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen LONP1 fusion protein Ag7196 相同性解析による交差性が予測される生物種
Full Name lon peptidase 1, mitochondrial
Calculated molecular weight 106 kDa
GenBank accession numberBC000235
Gene symbol LONP1
Gene ID (NCBI) 9361
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LONP1(Lon protease homolog, mitochondrial) is also named as LONP, LONHS, HLON, LON, PRSS15, PIM1, MGC1498 and belongs to the peptidase S16 family. It seems to play a major role in the elimination of oxidatively modified proteins in the mitochondrial matrix(PMID:18021745). LONP1, also a nuclearly encoded and mitochondrially located stress-responsive protease, is involved in heme-mediated ALAS-1 turnover(PMID:21659532). It recognizes specific surface determinants or folds, initiates proteolysis at solvent-accessible sites, and generates unfolded polypeptides that are then progressively degraded(PMID:15870080). LONP1 has some isoforms with the MW of 106 kDa, 100 kDa and 86 kDa.

Protocols

Product Specific Protocols
IF protocol for LONP1 antibody 83552-3-RRDownload protocol
FC protocol for LONP1 antibody 83552-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols