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CoraLite®594-conjugated LONP1 Monoclonal antibody

LONP1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1C6C12

Cat no : CL594-66043

Synonyms

Mitochondrial ATP-dependent protease Lon, LONP 1, LONP, LON, EC:3.4.21.53

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of C6 cells using CoraLite®594-conjugated LONP1 Monoclonal antibody (CL594-66043)

    IF Staining of C6 using CL594-66043

    Immunofluorescent analysis of (4% PFA) fixed C6 cells using CoraLite®594 LONP1 antibody (CL594-66043, Clone: 1C6C12 ) at dilution of 1:100. DAPI (blue).

  • Immunofluorescence (IF) / fluorescent staining of C6 cells using CoraLite®594-conjugated LONP1 Monoclonal antibody (CL594-66043)

    IF Staining of C6 using CL594-66043

    Immunofluorescent analysis of (4% PFA) fixed C6 cells using CoraLite®594 LONP1 antibody (CL594-66043, Clone: 1C6C12 ) at dilution of 1:100, CL488-Phalloidin (green).

  • Immunofluorescence (IF) / fluorescent staining of C6 cells using CoraLite®594-conjugated LONP1 Monoclonal antibody (CL594-66043)

    IF Staining of C6 using CL594-66043

    Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using CoraLite®594 LONP1 antibody (CL594-66043, Clone: 1C6C12 ) at dilution of 1:100, CL488-Phalloidin (green).

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Tested Applications

Positive IF/ICC detected inC6 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66043 targets LONP1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LONP1 fusion protein Ag7306 相同性解析による交差性が予測される生物種
Full Name lon peptidase 1, mitochondrial
Calculated molecular weight 106 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC000235
Gene symbol LONP1
Gene ID (NCBI) 9361
RRIDAB_2919942
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LONP1(Lon protease homolog, mitochondrial) is also named as LONP, LONHS, HLON, LON, PRSS15, PIM1, MGC1498 and belongs to the peptidase S16 family. It seems to play a major role in the elimination of oxidatively modified proteins in the mitochondrial matrix(PMID:18021745). LONP1, also a nuclearly encoded and mitochondrially located stress-responsive protease, is involved in heme-mediated ALAS-1 turnover(PMID:21659532). It recognizes specific surface determinants or folds, initiates proteolysis at solvent-accessible sites, and generates unfolded polypeptides that are then processively degraded(PMID:15870080).

Protocols

Product Specific Protocols
IF protocol for CL594 LONP1 antibody CL594-66043Download protocol
Standard Protocols
Click here to view our Standard Protocols