LPIN1 Monoclonal antibody
LPIN1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, Rat, Mouse and More (3)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
3G11B10
Cat no : 68124-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, Jurkat cells, MOLT-4 cells, K-562 cells |
| Positive IHC detected in | human liver cancer tissue, rat skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
Product Information
68124-1-Ig targets LPIN1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Rat, Mouse samples.
| Tested Reactivity | Human, Rat, Mouse |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | LPIN1 fusion protein Ag25813 相同性解析による交差性が予測される生物種 |
| Full Name | lipin 1 |
| Calculated molecular weight | 890 aa, 99 kDa |
| Observed molecular weight | 130 kDa |
| GenBank accession number | BC030537 |
| Gene symbol | LPIN1 |
| Gene ID (NCBI) | 23175 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LPIN1, also named as KIAA0188, belongs to the lipin family.It is a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis and therefore controls the metabolism of fatty acids at different levels. LPIN1 has a molecular weight of about 99 kDa but predicted bands around 130 kDa.(PMID:20231281, PMID:29765047).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LPIN1 antibody 68124-1-Ig | Download protocol |
| IHC protocol for LPIN1 antibody 68124-1-Ig | Download protocol |
| IF protocol for LPIN1 antibody 68124-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Eur J Pharmacol Exploring the effects of epigallocatechin gallate on lipid metabolism in the rat steatotic liver during normothermic machine perfusion: Insights from lipidomics and RNA sequencing | ||
Food Chem Toxicol Toosendanin induces hepatotoxicity via disrupting LXRα/Lipin1/SREBP1 mediated lipid metabolism |
