• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LPIN1 Monoclonal antibody, PBS Only

LPIN1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Rat, Mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G11B10

Cat no : 68124-1-PBS

Synonyms

DKFZp781P1796, KIAA0188, lipin 1, LPIN1, PAP1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using LPIN1 Monoclonal antibody (68124-1-Ig)

    WB analysis using 68124-1-Ig (same clone as 68124-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68124-1-Ig (LPIN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68124-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using LPIN1 Monoclonal antibody (68124-1-Ig)

    IHC staining of rat skeletal muscle using 68124-1-Ig (same clone as 68124-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 68124-1-Ig (LPIN1 antibody) at dilution of 1:1 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68124-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using LPIN1 Monoclonal antibody (68124-1-Ig)

    IHC staining of rat skeletal muscle using 68124-1-Ig (same clone as 68124-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 68124-1-Ig (LPIN1 antibody) at dilution of 1:1 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68124-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LPIN1 Monoclonal antibody (68124-1-Ig)

    IHC staining of human liver cancer using 68124-1-Ig (same clone as 68124-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68124-1-Ig (LPIN1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68124-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LPIN1 Monoclonal antibody (68124-1-Ig)

    IHC staining of human liver cancer using 68124-1-Ig (same clone as 68124-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68124-1-Ig (LPIN1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68124-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using LPIN1 Monoclonal antibody (68124-1-Ig)

    IF Staining of NIH/3T3 using 68124-1-Ig (same clone as 68124-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using LPIN1 antibody (68124-1-Ig, Clone: 3G11B10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68124-1-PBS in a different storage buffer formulation.

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68124-1-PBS targets LPIN1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Reactivity Human, Rat, Mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen LPIN1 fusion protein Ag25813 相同性解析による交差性が予測される生物種
Full Name lipin 1
Calculated molecular weight 890 aa, 99 kDa
Observed molecular weight130 kDa
GenBank accession numberBC030537
Gene symbol LPIN1
Gene ID (NCBI) 23175
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

LPIN1, also named as KIAA0188, belongs to the lipin family.It is a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis and therefore controls the metabolism of fatty acids at different levels. LPIN1 has a molecular weight of about 99 kDa but predicted bands around 130 kDa.(PMID:20231281, PMID:29765047).