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Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using LRMP Polyclonal antibody (19498-1-AP)

    WB analysis of Raji using 19498-1-AP (same clone as 19498-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 19498-1-AP (LRMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19498-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using LRMP Polyclonal antibody (19498-1-AP)

    WB analysis of Jurkat using 19498-1-AP (same clone as 19498-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19498-1-AP (LRMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19498-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using LRMP Polyclonal antibody (19498-1-AP)

    IHC staining of human spleen using 19498-1-AP (same clone as 19498-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 19498-1-AP (LRMP antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19498-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using LRMP Polyclonal antibody (19498-1-AP)

    IHC staining of human spleen using 19498-1-AP (same clone as 19498-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 19498-1-AP (LRMP antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19498-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using LRMP Polyclonal antibody (19498-1-AP)

    IF Staining of Jurkat using 19498-1-AP (same clone as 19498-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using LRMP antibody (19498-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 19498-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using LRMP Polyclonal antibody (19498-1-AP)

    FC experiment of Jurkat using 19498-1-AP (same clone as 19498-1-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.4 ug LRMP Polyclonal antibody (19498-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 19498-1-PBS in a different storage buffer formulation.

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LRMP Polyclonal antibody, PBS Only

LRMP Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 19498-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Protein Jaw1, Processed inositol 1,4,5-triphosphate receptor associated 2, Lymphoid-restricted membrane protein, JAW1, IRAG2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19498-1-PBS targets LRMP in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name lymphoid-restricted membrane protein
Calculated molecular weight 62 kDa
Observed molecular weight 70 kDa
GenBank accession numberNM_006152
Gene Symbol LRMP
Gene ID (NCBI) 4033
RRIDAB_10640430
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12912
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

LRMP, also named as JAW1, is a lymphoid restricted integral membrane protein that is localised to the cytoplasmic face of the endoplasmic reticulum.