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Validation Data Gallery

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  • Flow cytometry (FC) experiment of U-87 MG cells using Anti-Human LRP1 (5A6) (65233-1-Ig)

    FC experiment of U-87 MG using 65233-1-Ig

    1X10^6 U-87 MG cells were surface stained with 1 ug Anti-Human LRP1 (65233-1-Ig, Clone:5A6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 1 ug Control Antibody. Cells were not fixed.

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Anti-Human LRP1 (5A6)

LRP1 Monoclonal Antibody for FC
Cat No. 65233-1-Ig
Clone No.5A6

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

FC

Synonyms

A2MR, Alpha 2 macroglobulin receptor, APOER, Apolipoprotein E receptor, APR, CD91, FLJ16451, IGFBP3R, LRP, LRP 1, LRP1, LRPICD, TGFBR5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC detected inU-87 MG cells

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65233-1-Ig targets LRP1 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Full length native protein (purified) 相同性解析による交差性が予測される生物種
Full Name low density lipoprotein-related protein 1 (alpha-2-macroglobulin receptor)
Calculated molecular weight 505 kDa
GenBank accession numberBC045107
Gene Symbol LRP1
Gene ID (NCBI) 4035
RRIDAB_3084909
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
UNIPROT IDQ07954
Storage Buffer PBS with 0.09% sodium azide , pH 7.3
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

LRP1 (Prolow-density lipoprotein receptor-related protein 1), also known as A2MR, APOER and CD91, is a type I transmembrane protein that belongs to the LDLR family. LRP1 is synthesized as a transmembrane glycosylated precursor of 600 kDa, and is subsequently cleaved to generate a large 515-kDa N-terminal extracellular subunit and an 85-kDa C-terminal transmembrane subunit, which are noncovalently associated with one another (PMID: 2112085; 8546712). LRP1 is a multifunctional receptor involved in the clearance of a large number of diverse ligands and modulating various cellular processes. LRP1 is implicated in conditions such as atherosclerosis, cancer and neurodegenerative diseases (PMID: 28381441).

Protocols

Product Specific Protocols
FC protocol for LRP1 antibody 65233-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols