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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cardinal Red™ Anti-Human LRP1 (5A6)

LRP1 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

FC

Conjugate

Cardinal Red™ Fluorescent Dye

CloneNo.

5A6

Cat no : CR-65233

Synonyms

A2MR, Alpha 2 macroglobulin receptor, APOER, Apolipoprotein E receptor, APR, CD91, FLJ16451, IGFBP3R, LRP, LRP 1, LRP1, LRPICD, TGFBR5

Validation Data Gallery

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  • Flow cytometry (FC) experiment of U-87 MG cells using Cardinal Red™ Anti-Human LRP1 (5A6) (CR-65233)

    FC experiment of U-87 MG using CR-65233

    1X10^6 U-87 MG cells were surface stained with 5 ul Cardinal Red™ Anti-Human LRP1 (CR-65233, Clone:5A6), or Mouse IgG2b Isotype Control. Cells were not fixed.

  • Flow cytometry (FC) experiment of U-87 MG cells using Cardinal Red™ Anti-Human LRP1 (5A6) (CR-65233)

    FC experiment of U-87 MG using CR-65233

    1X10^6 U-87 MG cells were surface stained with 5 ul Cardinal Red™ Anti-Human LRP1 (CR-65233, Clone:5A6), or Mouse IgG2b Isotype Control. Cells were not fixed.

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Tested Applications

Positive FC detected inU-87 MG cells

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CR-65233 targets LRP1 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Full length native protein (purified) 相同性解析による交差性が予測される生物種
Full Name low density lipoprotein-related protein 1 (alpha-2-macroglobulin receptor)
Calculated molecular weight 505 kDa
GenBank accession numberBC045107
Gene symbol LRP1
Gene ID (NCBI) 4035
Conjugate Cardinal Red™ Fluorescent Dye
Excitation/Emission maxima wavelengths592 nm / 611 nm
Form Liquid
Purification MethodN/A
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

LRP1 (Prolow-density lipoprotein receptor-related protein 1), also known as A2MR, APOER and CD91, is a type I transmembrane protein that belongs to the LDLR family. LRP1 is synthesized as a transmembrane glycosylated precursor of 600 kDa, and is subsequently cleaved to generate a large 515-kDa N-terminal extracellular subunit and an 85-kDa C-terminal transmembrane subunit, which are noncovalently associated with one another (PMID: 2112085; 8546712). LRP1 is a multifunctional receptor involved in the clearance of a large number of diverse ligands and modulating various cellular processes. LRP1 is implicated in conditions such as atherosclerosis, cancer and neurodegenerative diseases (PMID: 28381441).

Protocols

Product Specific Protocols
FC protocol for Cardinal Red™ LRP1 antibody CR-65233Download protocol
Standard Protocols
Click here to view our Standard Protocols