Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 21175-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.
WB analysis using 21175-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 21175-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 21175-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 21175-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 21175-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 21175-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21175-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of rat kidney using 21175-1-AP
rat kidney tissue were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 21175-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 21175-1-AP
IP result of anti-LRPPRC (IP:21175-1-AP, 5ug; Detection:21175-1-AP 1:1000) with HeLa cells lysate 1200ug.
IHC staining of human normal colon using 21175-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 21175-1-AP (LRPPRC antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 21175-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 21175-1-AP (LRPPRC antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human heart using 21175-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 21175-1-AP (LRPPRC antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human kidney using 21175-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21175-1-AP (LRPPRC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 21175-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21175-1-AP (LRPPRC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human normal colon using 21175-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 21175-1-AP (LRPPRC antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of U2OS using 21175-1-AP
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 21175-1-AP (LRPPRC antibody), at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red) and DNA is stained by DAPI (blue).
IF Staining of HEK-293 using 21175-1-AP
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using LRPPRC antibody (21175-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of U2OS using 21175-1-AP
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 21175-1-AP (LRPPRC antibody), at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red) and DNA is stained by DAPI (blue).
IF Staining of HEK-293 using 21175-1-AP
Immunofluorescent analysis of 293 cells, using LRPPRC antibody 21175-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IF Staining of HeLa using 21175-1-AP
Immunofluorescent analysis of Hela cells, using LRPPRC antibody 21175-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IF Staining of HepG2 using 21175-1-AP
Immunofluorescent analysis of HepG2 cells, using LRPPRC antibody 21175-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IF Staining of HEK-293 using 21175-1-AP
Immunofluorescent analysis of HEK-293 cells using 21175-1-AP (LRPPRC antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

LRPPRC Polyclonal antibody

LRPPRC Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 21175-1-AP

Publications(38)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Synonyms

LRP130, LRP 130, Leucine-rich PPR motif-containing protein, mitochondrial, GP130, 130 kDa leucine-rich protein

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (38)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HCT 116 cells, mouse heart tissue, HepG2 cells, mouse skeletal muscle tissue, mouse kidney tissue, mouse liver tissue, HeLa cells, rat kidney tissue, rat liver tissue, rat heart tissue, PC-12 cells, SH-SY5Y cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human kidney tissue, human heart tissue, human normal colon **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	U2OS cells, HepG2 cells, HeLa cells, HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 31 publications below
IHC	See 6 publications below
IF	See 7 publications below
IP	See 1 publications below
CoIP	See 3 publications below
RIP	See 1 publications below

Product Information

21175-1-AP targets LRPPRC in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	LRPPRC fusion protein Ag15452 相同性解析による交差性が予測される生物種
Full Name	leucine-rich PPR-motif containing
Calculated molecular weight	1394 aa, 158 kDa
Observed molecular weight	130 kDa
GenBank accession number	BC050311
Gene Symbol	LRPPRC
Gene ID (NCBI)	10128
RRID	AB_10733879
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P42704
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

LRPPRC (also called LRP130 or GP130) is a 130-kDa RNA-binding protein of the pentatricopeptide repeat family. LRPPRC localizes primarily to the mitochondria where it binds to poly(A) mRNA. It plays a role in the translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. LRPPRC has also been shown to regulate nuclear gene transcription and to bind specific RNA molecules in both the nucleus and the cytoplasm. Mutations in the LRPPRC gene are associated with the French-Canadian type of Leigh syndrome.

Protocols

Product Specific Protocols
WB protocol for LRPPRC antibody 21175-1-AP	Download protocol
IHC protocol for LRPPRC antibody 21175-1-AP	Download protocol
IF protocol for LRPPRC antibody 21175-1-AP	Download protocol
IP protocol for LRPPRC antibody 21175-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IP	Nat Biotechnol Translation efficiency of mRNAs is increased by antisense oligonucleotides targeting upstream open reading frames. Authors - Xue-Hai Liang View Article
mouse	IF,WB,CoIP	Nat Commun Mitochondrial membrane proteins and VPS35 orchestrate selective removal of mtDNA Authors - Ayesha Sen View Article
human	WB,IF	Nat Commun A mosquito salivary protein promotes flavivirus transmission by activation of autophagy. Authors - Peng Sun View Article
human	WB	Genome Biol Balanced mitochondrial and cytosolic translatomes underlie the biogenesis of human respiratory complexes Authors - Iliana Soto KO Validated View Article
human	IHC,CoIP	Redox Biol LRPPRC regulates redox homeostasis via the circANKHD1/FOXM1 axis to enhance bladder urothelial carcinoma tumorigenesis. Authors - Wen-Su Wei View Article
human	WB	EMBO J Functional screen reveals essential roles of miR-27a/24 in differentiation of embryonic stem cells. Authors - Yanni Ma View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 21175-1-AP

WB analysis using 21175-1-AP

WB analysis of mouse heart using 21175-1-AP

WB analysis of HepG2 using 21175-1-AP

WB analysis of mouse skeletal muscle using 21175-1-AP

WB analysis of mouse kidney using 21175-1-AP

WB analysis of mouse liver using 21175-1-AP

WB analysis of HeLa using 21175-1-AP

WB analysis of rat kidney using 21175-1-AP

WB analysis of rat liver using 21175-1-AP

IP experiment of HeLa using 21175-1-AP

IHC staining of human normal colon using 21175-1-AP

IHC staining of human heart using 21175-1-AP

IHC staining of human heart using 21175-1-AP

IHC staining of human kidney using 21175-1-AP

IHC staining of human kidney using 21175-1-AP

IHC staining of human normal colon using 21175-1-AP

IF Staining of U2OS using 21175-1-AP

IF Staining of HEK-293 using 21175-1-AP

IF Staining of U2OS using 21175-1-AP

IF Staining of HEK-293 using 21175-1-AP

IF Staining of HeLa using 21175-1-AP

IF Staining of HepG2 using 21175-1-AP

IF Staining of HEK-293 using 21175-1-AP

LRPPRC Polyclonal antibody

LRPPRC Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Translation efficiency of mRNAs is increased by antisense oligonucleotides targeting upstream open reading frames.

Mitochondrial membrane proteins and VPS35 orchestrate selective removal of mtDNA

A mosquito salivary protein promotes flavivirus transmission by activation of autophagy.

Balanced mitochondrial and cytosolic translatomes underlie the biogenesis of human respiratory complexes

LRPPRC regulates redox homeostasis via the circANKHD1/FOXM1 axis to enhance bladder urothelial carcinoma tumorigenesis.

Functional screen reveals essential roles of miR-27a/24 in differentiation of embryonic stem cells.