Validation Data Gallery
Filter:
Tested Applications
| Positive IF/ICC detected in | HEK-293 cells |
| Positive FC (Intra) detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-21175 targets LRPPRC in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | LRPPRC fusion protein Ag15452 相同性解析による交差性が予測される生物種 |
| Full Name | leucine-rich PPR-motif containing |
| Calculated molecular weight | 1394 aa, 158 kDa |
| Observed molecular weight | 130 kDa |
| GenBank accession number | BC050311 |
| Gene Symbol | LRPPRC |
| Gene ID (NCBI) | 10128 |
| RRID | AB_3084049 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42704 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LRPPRC (also called LRP130 or GP130) is a 130-kDa RNA-binding protein of the pentatricopeptide repeat family. LRPPRC localizes primarily to the mitochondria where it binds to poly(A) mRNA. It plays a role in translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. LRPPRC has also been shown to regulate nuclear gene transcription and to bind specific RNA molecules in both the nucleus and the cytoplasm. Mutations in LRPPRC gene are associated with the French-Canadian type of Leigh syndrome.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 LRPPRC antibody CL488-21175 | Download protocol |
| FC protocol for CL Plus 488 LRPPRC antibody CL488-21175 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |