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LRPPRC Monoclonal antibody

LRPPRC Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3H7E1

Cat no : 67679-1-Ig

Synonyms

LRP130, LRP 130, Leucine-rich PPR motif-containing protein, mitochondrial, GP130, 3H7E1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LRPPRC Monoclonal antibody (67679-1-Ig)

    WB analysis using 67679-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67679-1-Ig (LRPPRC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using LRPPRC Monoclonal antibody (67679-1-Ig)

    WB analysis of HeLa using 67679-1-Ig

    WB result of LRPPRC antibody (67679-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LRPPRC transfected HeLa cells.

  • Western Blot (WB) analysis of U-251 cells using LRPPRC Monoclonal antibody (67679-1-Ig)

    WB analysis of U-251 using 67679-1-Ig

    U-251 cells were subjected to SDS PAGE followed by western blot with 67679-1-Ig (LRPPRC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using LRPPRC Monoclonal antibody (67679-1-Ig)

    IHC staining of human colon cancer using 67679-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using LRPPRC Monoclonal antibody (67679-1-Ig)

    IHC staining of human colon cancer using 67679-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using LRPPRC Monoclonal antibody (67679-1-Ig)

    IHC staining of human endometrial cancer using 67679-1-Ig

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using LRPPRC Monoclonal antibody (67679-1-Ig)

    IHC staining of human endometrial cancer using 67679-1-Ig

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LRPPRC Monoclonal antibody (67679-1-Ig)

    IHC staining of human liver cancer using 67679-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LRPPRC Monoclonal antibody (67679-1-Ig)

    IHC staining of human liver cancer using 67679-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using LRPPRC Monoclonal antibody (67679-1-Ig)

    IF Staining of HeLa using 67679-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LRPPRC Monoclonal antibody (67679-1-Ig)

    IF Staining of HepG2 using 67679-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using LRPPRC Monoclonal antibody (67679-1-Ig)

    IF Staining of HEK-293 using 67679-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). F-actin is stained using CoraLite®555-phalloidin (red) and DNA is stained by DAPI (blue).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LRPPRC Monoclonal antibody (67679-1-Ig)

    IF Staining of HepG2 using 67679-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using LRPPRC Monoclonal antibody (67679-1-Ig)

    IF Staining of A431 using 67679-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inU2OS cells, U-251 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman colon cancer tissue, human endometrial cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HEK-293 cells, HepG2 cells, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67679-1-Ig targets LRPPRC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen LRPPRC fusion protein Ag30261 相同性解析による交差性が予測される生物種
Full Name leucine-rich PPR-motif containing
Calculated molecular weight 1394 aa, 158 kDa
Observed molecular weight130 kDa
GenBank accession numberBC050311
Gene symbol LRPPRC
Gene ID (NCBI) 10128
RRIDAB_2882872
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LRPPRC (also called LRP130 or GP130) is a 130-kDa RNA-binding protein of the pentatricopeptide repeat family. LRPPRC localizes primarily to the mitochondria where it binds to poly(A) mRNA. It plays a role in translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. LRPPRC has also been shown to regulate nuclear gene transcription and to bind specific RNA molecules in both the nucleus and the cytoplasm. Mutations in LRPPRC gene are associated with the French-Canadian type of Leigh syndrome.

Protocols

Product Specific Protocols
WB protocol for LRPPRC antibody 67679-1-IgDownload protocol
IHC protocol for LRPPRC antibody 67679-1-IgDownload protocol
IF protocol for LRPPRC antibody 67679-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Front Immunol

Expression and clinical significance of interleukin-6 pathway in cholangiocarcinoma

Authors - Dongqing Gu
View Article
humanWB

Front Genet

RNA methylation pattern and immune microenvironment characteristics mediated by m6A regulator in ischemic stroke

Authors - Kejuan Jia
View Article