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  • Featured Product
  • KD/KO Validated

LRPPRC Monoclonal antibody, PBS Only

LRPPRC Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3H7E1

Cat no : 67679-1-PBS

Synonyms

LRP130, LRP 130, Leucine-rich PPR motif-containing protein, mitochondrial, GP130, 3H7E1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LRPPRC Monoclonal antibody (67679-1-Ig)

    WB analysis using 67679-1-Ig (same clone as 67679-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67679-1-Ig (LRPPRC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using LRPPRC Monoclonal antibody (67679-1-Ig)

    WB analysis of HeLa using 67679-1-Ig (same clone as 67679-1-PBS)

    WB result of LRPPRC antibody (67679-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LRPPRC transfected HeLa cells. This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-251 cells using LRPPRC Monoclonal antibody (67679-1-Ig)

    WB analysis of U-251 using 67679-1-Ig (same clone as 67679-1-PBS)

    U-251 cells were subjected to SDS PAGE followed by western blot with 67679-1-Ig (LRPPRC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using LRPPRC Monoclonal antibody (67679-1-Ig)

    IHC staining of human colon cancer using 67679-1-Ig (same clone as 67679-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using LRPPRC Monoclonal antibody (67679-1-Ig)

    IHC staining of human colon cancer using 67679-1-Ig (same clone as 67679-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using LRPPRC Monoclonal antibody (67679-1-Ig)

    IHC staining of human endometrial cancer using 67679-1-Ig (same clone as 67679-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using LRPPRC Monoclonal antibody (67679-1-Ig)

    IHC staining of human endometrial cancer using 67679-1-Ig (same clone as 67679-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LRPPRC Monoclonal antibody (67679-1-Ig)

    IHC staining of human liver cancer using 67679-1-Ig (same clone as 67679-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LRPPRC Monoclonal antibody (67679-1-Ig)

    IHC staining of human liver cancer using 67679-1-Ig (same clone as 67679-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LRPPRC Monoclonal antibody (67679-1-Ig)

    IF Staining of HepG2 using 67679-1-Ig (same clone as 67679-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using LRPPRC Monoclonal antibody (67679-1-Ig)

    IF Staining of HeLa using 67679-1-Ig (same clone as 67679-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using LRPPRC Monoclonal antibody (67679-1-Ig)

    IF Staining of HEK-293 using 67679-1-Ig (same clone as 67679-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). F-actin is stained using CoraLite®555-phalloidin (red) and DNA is stained by DAPI (blue). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LRPPRC Monoclonal antibody (67679-1-Ig)

    IF Staining of HepG2 using 67679-1-Ig (same clone as 67679-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using LRPPRC Monoclonal antibody (67679-1-Ig)

    IF Staining of A431 using 67679-1-Ig (same clone as 67679-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67679-1-PBS targets LRPPRC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen LRPPRC fusion protein Ag30261 相同性解析による交差性が予測される生物種
Full Name leucine-rich PPR-motif containing
Calculated molecular weight 1394 aa, 158 kDa
Observed molecular weight130 kDa
GenBank accession numberBC050311
Gene symbol LRPPRC
Gene ID (NCBI) 10128
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

LRPPRC (also called LRP130 or GP130) is a 130-kDa RNA-binding protein of the pentatricopeptide repeat family. LRPPRC localizes primarily to the mitochondria where it binds to poly(A) mRNA. It plays a role in translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. LRPPRC has also been shown to regulate nuclear gene transcription and to bind specific RNA molecules in both the nucleus and the cytoplasm. Mutations in LRPPRC gene are associated with the French-Canadian type of Leigh syndrome.