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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LRRC47 Recombinant antibody, PBS Only

LRRC47 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240741A6

Cat no : 83693-3-PBS

Synonyms

Leucine-rich repeat-containing protein 47, KIAA1185, 240741A6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LRRC47 Recombinant antibody (83693-3-RR)

    WB analysis using 83693-3-RR (same clone as 83693-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83693-3-RR (LRRC47 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83693-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using LRRC47 Recombinant antibody (83693-3-RR)

    IF Staining of L02 using 83693-3-RR (same clone as 83693-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed L02 cells using LRRC47 antibody (83693-3-RR, Clone: 240741A6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83693-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U2OS cells using LRRC47 Recombinant antibody (83693-3-RR)

    FC experiment of U2OS using 83693-3-RR (same clone as 83693-3-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug LRRC47 Recombinant antibody (83693-3-RR, Clone:240741A6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83693-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83693-3-RR

    Affinity and Kinetic Characterization of 83693-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83693-3-RR against Human LRRC47 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83693-3-PBS is LRRC47 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen LRRC47 fusion protein Ag19736 相同性解析による交差性が予測される生物種
Full Name leucine rich repeat containing 47
Calculated molecular weight 583 aa, 63 kDa
Observed molecular weight63 kDa
GenBank accession numberBC031301
Gene symbol LRRC47
Gene ID (NCBI) 57470
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

LRRC47 enables RNA binding activity. It is predicted to be involved in phenylalanyl-tRNA aminoacylation.