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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LRRC47 Recombinant antibody

LRRC47 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240741A6

Cat no : 83693-3-RR

Synonyms

Leucine-rich repeat-containing protein 47, KIAA1185, 240741A6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LRRC47 Recombinant antibody (83693-3-RR)

    WB analysis using 83693-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83693-3-RR (LRRC47 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using LRRC47 Recombinant antibody (83693-3-RR)

    IF Staining of L02 using 83693-3-RR

    Immunofluorescent analysis of (4% PFA) fixed L02 cells using LRRC47 antibody (83693-3-RR, Clone: 240741A6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of U2OS cells using LRRC47 Recombinant antibody (83693-3-RR)

    FC experiment of U2OS using 83693-3-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug LRRC47 Recombinant antibody (83693-3-RR, Clone:240741A6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83693-3-RR

    Affinity and Kinetic Characterization of 83693-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83693-3-RR against Human LRRC47 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inDaudi cells, U-87 MG cells, L02 cells
Positive IF/ICC detected inL02 cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83693-3-RR is LRRC47 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen LRRC47 fusion protein Ag19736 相同性解析による交差性が予測される生物種
Full Name leucine rich repeat containing 47
Calculated molecular weight 583 aa, 63 kDa
Observed molecular weight63 kDa
GenBank accession numberBC031301
Gene symbol LRRC47
Gene ID (NCBI) 57470
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LRRC47 enables RNA binding activity. It is predicted to be involved in phenylalanyl-tRNA aminoacylation.

Protocols

Product Specific Protocols
WB protocol for LRRC47 antibody 83693-3-RRDownload protocol
IF protocol for LRRC47 antibody 83693-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols