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Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated LSM12 Monoclonal ant (CL488-67029)

    FC experiment of HeLa using CL488-67029

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated LSM12 Monoclonal antibody (CL488-67029, Clone:2E3G3)(red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  • KD/KO Validated

CoraLite® Plus 488-conjugated LSM12 Monoclonal antibody

LSM12 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67029
Clone No.2E3G3

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

FC (Intra)

Synonyms

Protein LSM12, 2E3G3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67029 targets LSM12 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen LSM12 fusion protein Ag28782 相同性解析による交差性が予測される生物種
Full Name LSM12 homolog (S. cerevisiae)
Observed molecular weight 22 kDa
GenBank accession numberBC044587
Gene Symbol LSM12
Gene ID (NCBI) 124801
RRIDAB_2934514
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ3MHD2
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 LSM12 antibody CL488-67029Download protocol
Standard Protocols
Click here to view our Standard Protocols