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  • Featured Product
  • KD/KO Validated

LTF Polyclonal antibody, PBS Only

LTF Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10933-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LTF Polyclonal antibody (10933-1-AP)

    WB analysis using 10933-1-AP (same clone as 10933-1-PBS)

    human saliva were subjected to SDS PAGE followed by western blot with 10933-1-AP (Lactoferrin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10933-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using LTF Polyclonal antibody (10933-1-AP)

    WB analysis of HEK-293 using 10933-1-AP (same clone as 10933-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10933-1-AP (Lactoferrin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10933-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human pancreas tissue using LTF Polyclonal antibody (10933-1-AP)

    WB analysis of human pancreas using 10933-1-AP (same clone as 10933-1-PBS)

    human pancreas tissue were subjected to SDS PAGE followed by western blot with 10933-1-AP (Lactoferrin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10933-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using LTF Polyclonal antibody (10933-1-AP)

    IP experiment of HeLa using 10933-1-AP (same clone as 10933-1-PBS)

    IP result of anti-LTF (IP:10933-1-AP, 4ug; Detection:10933-1-AP 1:600) with HeLa cells lysate 4000ug. This data was developed using the same antibody clone with 10933-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using LTF Polyclonal antibody (10933-1-AP)

    IHC staining of human liver using 10933-1-AP (same clone as 10933-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 10933-1-AP (Lactoferrin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10933-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using LTF Polyclonal antibody (10933-1-AP)

    IHC staining of human liver using 10933-1-AP (same clone as 10933-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 10933-1-AP (Lactoferrin antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10933-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using LTF Polyclonal antibody (10933-1-AP)

    IHC staining of human prostate hyperplasia using 10933-1-AP (same clone as 10933-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10933-1-AP (Lactoferrin antibody at dilution of 1:50(under 10x lens). This data was developed using the same antibody clone with 10933-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using LTF Polyclonal antibody (10933-1-AP)

    IHC staining of human prostate hyperplasia using 10933-1-AP (same clone as 10933-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10933-1-AP (Lactoferrin antibody at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 10933-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10933-1-PBS targets LTF in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LTF fusion protein Ag1379 相同性解析による交差性が予測される生物種
Full Name lactotransferrin
Calculated molecular weight 80 kDa
Observed molecular weight 30,51,80 kDa
GenBank accession numberBC015822
Gene symbol LTF
Gene ID (NCBI) 4057
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

LTF(Lactotransferrin) is also named as GIG12, LF and belongs to the transferrin family. It is an iron binding transport protein which can bind two atoms of ferric iron in association with the binding of an anion, usually bicarbonate. LTF is also an important protein component of the innate immune system that is broadly distributed within the body fluids. It can contribute to the activation of both the innate and adaptive immune responses by promoting the recruitment of leukocytes and activation of dendritic cells(PMID:18453607). This protein has 2 isoforms produced by alternative promoter usage. The human LTF can be isolated two fragments, an N-terminal tryptic fragment having an MW of 30 kDa and a C-terminal tryptic fragment having an MW of 50 kDa by a mild tryptic digest(PMID:3790094).