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  • Featured Product
  • KD/KO Validated

LY6D Recombinant antibody, PBS Only

LY6D Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4F12

Cat no : 82094-1-PBS

Synonyms

Lymphocyte antigen 6D, Ly-6D, Ly 6D, E48 antigen, E48

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using LY6D Recombinant antibody (82094-1-RR)

    WB analysis of A431 using 82094-1-RR (same clone as 82094-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 82094-1-RR (LY6D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82094-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using LY6D Recombinant antibody (82094-1-RR)

    WB analysis of A431 using 82094-1-RR (same clone as 82094-1-PBS)

    WB result of LY6D antibody (82094-1-RR; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LY6D transfected A431 cells. This data was developed using the same antibody clone with 82094-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using LY6D Recombinant antibody (82094-1-RR)

    IHC staining of human skin cancer using 82094-1-RR (same clone as 82094-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 82094-1-RR (LY6D antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82094-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using LY6D Recombinant antibody (82094-1-RR)

    IHC staining of human skin cancer using 82094-1-RR (same clone as 82094-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 82094-1-RR (LY6D antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82094-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82094-1-PBS targets LY6D in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen LY6D fusion protein Ag10714 相同性解析による交差性が予測される生物種
Full Name lymphocyte antigen 6 complex, locus D
Calculated molecular weight 128 aa, 13 kDa
Observed molecular weight 13-14 kDa
GenBank accession numberBC031330
Gene symbol LY6D
Gene ID (NCBI) 8581
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

LY6D, also named as E48 and ThB, may act as a specification marker at earliest stage specification of lymphocytes between B- and T-cell development. It marks the earliest stage of B-cell specification.