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  • Featured Product
  • KD/KO Validated

LY6D Recombinant antibody

LY6D Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4F12

Cat no : 82094-1-RR

Synonyms

Lymphocyte antigen 6D, Ly-6D, Ly 6D, E48 antigen, E48

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using LY6D Recombinant antibody (82094-1-RR)

    WB analysis of A431 using 82094-1-RR

    A431 cells were subjected to SDS PAGE followed by western blot with 82094-1-RR (LY6D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using LY6D Recombinant antibody (82094-1-RR)

    WB analysis of A431 using 82094-1-RR

    WB result of LY6D antibody (82094-1-RR; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LY6D transfected A431 cells.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using LY6D Recombinant antibody (82094-1-RR)

    IHC staining of human skin cancer using 82094-1-RR

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 82094-1-RR (LY6D antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using LY6D Recombinant antibody (82094-1-RR)

    IHC staining of human skin cancer using 82094-1-RR

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 82094-1-RR (LY6D antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inA431 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82094-1-RR targets LY6D in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen LY6D fusion protein Ag10714 相同性解析による交差性が予測される生物種
Full Name lymphocyte antigen 6 complex, locus D
Calculated molecular weight 128 aa, 13 kDa
Observed molecular weight 13-14 kDa
GenBank accession numberBC031330
Gene symbol LY6D
Gene ID (NCBI) 8581
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LY6D, also named as E48 and ThB, may act as a specification marker at earliest stage specification of lymphocytes between B- and T-cell development. It marks the earliest stage of B-cell specification.

Protocols

Product Specific Protocols
WB protocol for LY6D antibody 82094-1-RRDownload protocol
IHC protocol for LY6D antibody 82094-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols