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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 750-conjugated LY6D Recombinant antibody

LY6D Recombinant Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

4F12

Cat no : CL750-82094

Synonyms

Lymphocyte antigen 6D, Ly-6D, Ly 6D, E48 antigen, E48

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 750-conjugated LY6D Recombinant ant (CL750-82094)

    WB analysis using CL750-82094

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-82094 (LY6D antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of U2OS cells using CoraLite® Plus 750-conjugated LY6D Recombinant ant (CL750-82094)

    FC experiment of U2OS using CL750-82094

    1x10^6 U2OS cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated LY6D Recombinant antibody (CL750-82094, Clone:4F12)(red), or 0.4 ug Isotype Control (CL750-3000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inA431 cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-82094 targets LY6D in WB applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen LY6D fusion protein Ag10714 相同性解析による交差性が予測される生物種
Full Name lymphocyte antigen 6 complex, locus D
Calculated molecular weight 128 aa, 13 kDa
Observed molecular weight 13-14 kDa
GenBank accession numberBC031330
Gene symbol LY6D
Gene ID (NCBI) 8581
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission maxima wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LY6D, also named as E48 and ThB, may act as a specification marker at earliest stage specification of lymphocytes between B- and T-cell development. It marks the earliest stage of B-cell specification.

Protocols

Product Specific Protocols
WB protocol for CL Plus 750 LY6D antibody CL750-82094Download protocol
Standard Protocols
Click here to view our Standard Protocols