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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LZTFL1 Monoclonal antibody

LZTFL1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2C1F6

Cat no : 68281-1-Ig

Synonyms

Leucine zipper transcription factor-like protein 1, 2C1F6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LZTFL1 Monoclonal antibody (68281-1-Ig)

    WB analysis using 68281-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68281-1-Ig (LZTFL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using LZTFL1 Monoclonal antibody (68281-1-Ig)

    IF Staining of Jurkat using 68281-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using LZTFL1 antibody (68281-1-Ig, Clone: 2C1F6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of Jurkat cells using LZTFL1 Monoclonal antibody (68281-1-Ig)

    FC experiment of Jurkat using 68281-1-Ig

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human LZTFL1 (68281-1-Ig, Clone:2C1F6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHCT 116 cells, LNCaP cells, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68281-1-Ig targets LZTFL1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen LZTFL1 fusion protein Ag10733 相同性解析による交差性が予測される生物種
Full Name leucine zipper transcription factor-like 1
Calculated molecular weight 299 aa, 35 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC025988
Gene symbol LZTFL1
Gene ID (NCBI) 54585
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LZTFL1 belongs to the LZTFL1 family. It is an important regulator of BBSome ciliary trafficking and hedgehog signaling. LZTFL1 regulates Ciliary Trafficking of the BBSome and Smoothened.(PMID:22072986)

Protocols

Product Specific Protocols
WB protocol for LZTFL1 antibody 68281-1-IgDownload protocol
IF protocol for LZTFL1 antibody 68281-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols