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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LZTFL1 Monoclonal antibody, PBS Only

LZTFL1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C1F6

Cat no : 68281-1-PBS

Synonyms

Leucine zipper transcription factor-like protein 1, 2C1F6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LZTFL1 Monoclonal antibody (68281-1-Ig)

    WB analysis using 68281-1-Ig (same clone as 68281-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68281-1-Ig (LZTFL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68281-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using LZTFL1 Monoclonal antibody (68281-1-Ig)

    IF Staining of Jurkat using 68281-1-Ig (same clone as 68281-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using LZTFL1 antibody (68281-1-Ig, Clone: 2C1F6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68281-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using LZTFL1 Monoclonal antibody (68281-1-Ig)

    FC experiment of Jurkat using 68281-1-Ig (same clone as 68281-1-PBS)

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human LZTFL1 (68281-1-Ig, Clone:2C1F6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68281-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68281-1-PBS targets LZTFL1 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen LZTFL1 fusion protein Ag10733 相同性解析による交差性が予測される生物種
Full Name leucine zipper transcription factor-like 1
Calculated molecular weight 299 aa, 35 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC025988
Gene symbol LZTFL1
Gene ID (NCBI) 54585
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

LZTFL1 belongs to the LZTFL1 family. It is an important regulator of BBSome ciliary trafficking and hedgehog signaling. LZTFL1 regulates Ciliary Trafficking of the BBSome and Smoothened.(PMID:22072986)