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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Lamin A/C Recombinant antibody (81042-1-RR)

    WB analysis of HeLa using 81042-1-RR (same clone as 81042-1-PBS)

    WB result of Lamin A/C antibody (81042-1-RR; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Lamin A/C transfected HeLa cells. This data was developed using the same antibody clone with 81042-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Lamin A/C Recombinant antibody (81042-1-RR)

    WB analysis using 81042-1-RR (same clone as 81042-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81042-1-RR (Lamin A/C antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81042-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Lamin A/C Recombinant antibody (81042-1-RR)

    WB analysis using 81042-1-RR (same clone as 81042-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81042-1-RR (Lamin A/C antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81042-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Lamin A/C Recombinant antibody (81042-1-RR)

    IHC staining of mouse heart using 81042-1-RR (same clone as 81042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 81042-1-RR (Lamin A/C antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81042-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Lamin A/C Recombinant antibody (81042-1-RR)

    IHC staining of mouse heart using 81042-1-RR (same clone as 81042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 81042-1-RR (Lamin A/C antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81042-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Lamin A/C Recombinant antibody (81042-1-RR)

    IHC staining of human colon using 81042-1-RR (same clone as 81042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 81042-1-RR (Lamin A/C antibody) at dilution of 1:12000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81042-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Lamin A/C Recombinant antibody (81042-1-RR)

    IHC staining of human colon using 81042-1-RR (same clone as 81042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 81042-1-RR (Lamin A/C antibody) at dilution of 1:12000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81042-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Lamin A/C Recombinant antibody (81042-1-RR)

    IF Staining of HepG2 using 81042-1-RR (same clone as 81042-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Lamin A/C antibody (81042-1-RR, Clone: 5I16 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81042-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81042-1-RR

    Affinity and Kinetic Characterization of 81042-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81042-1-RR against Human Lamin A/C were performed. The affinity constant is 92.9 pM.

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  • Featured Product
  • KD/KO Validated

Lamin A/C Recombinant antibody, PBS Only

Lamin A/C Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 81042-1-PBS
Clone No.5I16

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

LaminA/C, LMNA, LMN1, Lamin-A/C, lamin A

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81042-1-PBS targets Lamin A/C in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Lamin A/C fusion protein Ag0408 相同性解析による交差性が予測される生物種
Full Name lamin A/C
Calculated molecular weight 65 kDa
Observed molecular weight65-70 kDa
GenBank accession numberBC003162
Gene Symbol Lamin A/C
Gene ID (NCBI) 4000
ENSEMBL Gene IDENSG00000160789
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02545
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Lamin A/C is also named as LMNA, or LMN1. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. The lack of lamin A/C can be as a novel marker for undifferentiated embryonic stem cells and lamin A/C expression is as an early indicator of differentiation (PMID: 16179429). Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. This protein has 4 isoforms produced by alternative splicing with the molecular weight of 74 kDa, 65 kDa, 70 kDa and 64 kDa. This antibody can recognize 4 isoforms of Lamin A/C.