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Lamin A/C Recombinant antibody
Lamin A/C Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
5I16
Cat no : 81042-1-RR
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, RAW 264.7 cells, HeLa cells, HUVEC cells, A431 cells, SKOV-3 cells, HSC-T6 cells, NIH/3T3 cells, C6 cells |
| Positive IHC detected in | mouse heart tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:3000-1:12000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
81042-1-RR targets Lamin A/C in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Lamin A/C fusion protein Ag0408 相同性解析による交差性が予測される生物種 |
| Full Name | lamin A/C |
| Calculated molecular weight | 65 kDa |
| Observed molecular weight | 65-70 kDa |
| GenBank accession number | BC003162 |
| Gene symbol | Lamin A/C |
| Gene ID (NCBI) | 4000 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Lamin A/C is also named as LMNA, or LMN1. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. The lack of lamin A/C can be as a novel marker for undifferentiated embryonic stem cells and lamin A/C expression is as an early indicator of differentiation (PMID: 16179429). Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. This protein has 4 isoforms produced by alternative splicing with the molecular weight of 74 kDa, 65 kDa, 70 kDa and 64 kDa. This antibody can recognize 4 isoforms of Lamin A/C.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Lamin A/C antibody 81042-1-RR | Download protocol |
| IHC protocol for Lamin A/C antibody 81042-1-RR | Download protocol |
| IF protocol for Lamin A/C antibody 81042-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Mol Sci Inflammation and Fibrosis in Progeria: Organ-Specific Responses in an HGPS Mouse Model |
