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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Lamin B2 Recombinant antibody

Lamin B2 Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230184C7

Cat no : CL488-82801-4

Synonyms

LMNB2, LMN2, Lamin-B2, LAMB2, 230184C7

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated Lamin B2 Recombinant (CL488-82801-4)

    FC experiment of HepG2 using CL488-82801-4

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Lamin B2 Recombinant Antibody (CL488-82801-4, Clone:230184C7)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82801-4 targets Lamin B2 in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Lamin B2 fusion protein Ag1335 相同性解析による交差性が予測される生物種
Full Name lamin B2
Calculated molecular weight 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC006551
Gene symbol Lamin B2
Gene ID (NCBI) 84823
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Lamins are nuclear membrane structural components that are important in structural integrity of the nucleus and may also interact with chromatin (PMID: 33033404). Research studies show that lamin B2 knockout mice exhibit neuronal developmental defects and that both proteins are essential for typical brain development (PMID: 20145110). Mutations in Lamin B2 can result in a susceptibility to developing acquired partial lipodystrophy, a rare disorder characterized by the progressive loss of subcutaneous fat in a bilaterally symmetrical fashion (PMID: 16826530).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 Lamin B2 antibody CL488-82801-4Download protocol
Standard Protocols
Click here to view our Standard Protocols