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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Rat Leptin Recombinant antibody, PBS Only

Leptin Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240368E6

Cat no : 83655-3-PBS

Synonyms

Lep, OB, 240368E6

Validation Data Gallery

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  • Western Blot (WB) analysis of rat adipose tissue using Leptin Recombinant antibody (83655-3-RR)

    WB analysis of rat adipose using 83655-3-RR (same clone as 83655-3-PBS)

    Rat adipose tissue was subjected to SDS PAGE followed by western blot with 83655-3-RR (Leptin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83655-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Leptin Recombinant antibody (83655-3-RR)

    IHC staining of human placenta using 83655-3-RR (same clone as 83655-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83655-3-RR (Leptin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83655-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Leptin Recombinant antibody (83655-3-RR)

    IHC staining of human placenta using 83655-3-RR (same clone as 83655-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83655-3-RR (Leptin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83655-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83655-3-RR

    Affinity and Kinetic Characterization of 83655-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83655-3-RR against Rat Leptin were performed. The affinity constant is 3.54 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83655-3-PBS is Leptin Fusion Protein expressed in E. coli.

Tested Reactivity rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name leptin
Calculated molecular weight19 kDa
Observed molecular weight 15-16 kDa
GenBank accession numberNM_013076.3
Gene symbol Leptin
Gene ID (NCBI) 25608
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Leptin is a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. Leptin is the most critical hormone in the homeostatic regulation of energy balance among those so far discovered. Leptin primarily acts on the neurons of the mediobasal part of hypothalamus to regulate food intake, thermogenesis, and the blood glucose level. Leptin also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. Leptin has also been linked to type 2 diabetes mellitus development.