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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Leptin Recombinant antibody

Leptin Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240368E6

Cat no : 83655-3-RR

Synonyms

Lep, OB, 240368E6

Validation Data Gallery

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  • Western Blot (WB) analysis of rat adipose tissue using Leptin Recombinant antibody (83655-3-RR)

    WB analysis of rat adipose using 83655-3-RR

    Rat adipose tissue was subjected to SDS PAGE followed by western blot with 83655-3-RR (Leptin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Leptin Recombinant antibody (83655-3-RR)

    IHC staining of human placenta using 83655-3-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83655-3-RR (Leptin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using Leptin Recombinant antibody (83655-3-RR)

    IHC staining of human placenta using 83655-3-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83655-3-RR (Leptin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83655-3-RR

    Affinity and Kinetic Characterization of 83655-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83655-3-RR against Rat Leptin were performed. The affinity constant is 3.54 nM.

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Tested Applications

Positive WB detected inrat adipose tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83655-3-RR is Leptin Fusion Protein expressed in E. coli.

Tested Reactivity rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name leptin
Calculated molecular weight19 kDa
Observed molecular weight 15-16 kDa
GenBank accession numberNM_013076.3
Gene symbol Leptin
Gene ID (NCBI) 25608
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Leptin is a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. Leptin is the most critical hormone in the homeostatic regulation of energy balance among those so far discovered. Leptin primarily acts on the neurons of the mediobasal part of hypothalamus to regulate food intake, thermogenesis, and the blood glucose level. Leptin also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. Leptin has also been linked to type 2 diabetes mellitus development.

Protocols

Product Specific Protocols
WB protocol for Leptin antibody 83655-3-RRDownload protocol
IHC protocol for Leptin antibody 83655-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols