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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Lipocalin-2/NGAL Recombinant antibody (82841-1-RR)

    WB analysis using 82841-1-RR (same clone as 82841-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82841-1-RR (Lipocalin-2/NGAL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82841-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-29 cells using Lipocalin-2/NGAL Recombinant antibody (82841-1-RR)

    WB analysis of HT-29 using 82841-1-RR (same clone as 82841-1-PBS)

    WB result of NGAL antibody (82841-1-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-NGAL transfected HT-29 cells. This data was developed using the same antibody clone with 82841-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using Lipocalin-2/NGAL Recombinant antibody (82841-1-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 82841-1-RR (same clone as 82841-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82841-1-RR (NGAL antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82841-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82841-1-RR

    Affinity and Kinetic Characterization of 82841-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82841-1-RR against Human NGAL were performed. The affinity constant is 0.776 nM.

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  • KD/KO Validated

Lipocalin-2/NGAL Recombinant antibody, PBS Only

Lipocalin-2/NGAL Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 82841-1-PBS
Clone No.2G1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

LCN2, lipocalin 2, NGAL, Lcn 2, HNL

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82841-1-PBS targets Lipocalin-2/NGAL in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name lipocalin 2
Calculated molecular weight23kd
Observed molecular weight22-25 kDa
GenBank accession numberNP_005555.2
Gene Symbol NGAL/LCN2
Gene ID (NCBI) 3934
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP80188
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Lipocalin-2 (LCN-2) is a adipocytokine also referred to as neutrophil gelatinase-associated lipocalin (NGAL). Lipocalin-2 is a circulatory protein responsible for the transportation of small and hydrophobic molecules to target organs. Lipocalin-2 is used as a biomarker for acute and chronic renal injury. It is present in a large variety of cells including neutrophil, hepatocytes, lung, bone marrow, adipose tissue, macrophages, thymus, non-neoplastic breast duct, prostate, and renal cells. Different functions have been associated with Lipocalin-2, including antibacterial, anti-inflammatory, and protection against cell and tissue stress (PMID:34463264).