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  • Featured Product
  • KD/KO Validated

NGAL Recombinant antibody

NGAL Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2G1

Cat no : 82841-1-RR

Synonyms

LCN2, lipocalin 2, Lcn 2, HNL, 2G1

Validation Data Gallery

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  • Western Blot (WB) analysis of HT-29 cells using NGAL Recombinant antibody (82841-1-RR)

    WB analysis of HT-29 using 82841-1-RR

    WB result of NGAL antibody (82841-1-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-NGAL transfected HT-29 cells.

  • Western Blot (WB) analysis of various lysates using NGAL Recombinant antibody (82841-1-RR)

    WB analysis using 82841-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82841-1-RR (Lipocalin-2/NGAL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using NGAL Recombinant antibody (82841-1-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 82841-1-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82841-1-RR (NGAL antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82841-1-RR

    Affinity and Kinetic Characterization of 82841-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82841-1-RR against Human NGAL were performed. The affinity constant is 0.776 nM.

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Tested Applications

Positive WB detected inhuman saliva, HT-29 cells, SW480 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82841-1-RR targets NGAL in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name lipocalin 2
Calculated molecular weight23kd
Observed molecular weight22-25 kDa
GenBank accession numberNP_005555.2
Gene symbol NGAL/LCN2
Gene ID (NCBI) 3934
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Lipocalin-2 (LCN-2) is a adipocytokine also referred to as neutrophil gelatinase-associated lipocalin (NGAL). Lipocalin-2 is a circulatory protein responsible for the transportation of small and hydrophobic molecules to target organs. Lipocalin-2 is used as a biomarker for acute and chronic renal injury. It is present in a large variety of cells including neutrophil, hepatocytes, lung, bone marrow, adipose tissue, macrophages, thymus, non-neoplastic breast duct, prostate, and renal cells. Different functions have been associated with Lipocalin-2, including antibacterial, anti-inflammatory, and protection against cell and tissue stress (PMID:34463264).

Protocols

Product Specific Protocols
WB protocol for NGAL antibody 82841-1-RRDownload protocol
IHC protocol for NGAL antibody 82841-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols