Validation Data Gallery
Tested Applications
| Positive FC detected in | mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-65140 targets Ly-6G/Ly-6C (Gr-1) in FC applications and shows reactivity with Mouse samples.
| Tested Reactivity | Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Raised against granulocytes of mouse origin 相同性解析による交差性が予測される生物種 |
| Full Name | lymphocyte antigen 6 complex, locus G |
| GenBank accession number | X70920 |
| Gene Symbol | Ly-6G |
| Gene ID (NCBI) | 546644 |
| RRID | AB_2934714 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P35461 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Ly-6G (lymphocyte antigen 6 complex, locus G), also known as Gr-1, is a 21-25 kDa, glycosylphosphatidylinositol-anchored protein expressed on myeloid lineage cells in mouse bone marrow (PMID: 8360469). The expression of Ly-6G increases on neutrophils as they differentiate from immature cells in the bone marrow to mature cells in the blood and spleen (PMID: 8890901). Antibodies targeting Ly6G (RB6-8C5 or 1A8) are commonly used in studies aimed at identifying the role of neutrophils (PMID: 23543767). The RB6-8C5 mAb has been reported to cross-react with Ly-6C on cells expressing Ly-6C (PMID: 8360469).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL594 Ly-6G/Ly-6C (Gr-1) antibody CL594-65140 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |