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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using MAD2L2 Polyclonal antibody (12683-1-AP)

    WB analysis of human brain using 12683-1-AP (same clone as 12683-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12683-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using MAD2L2 Polyclonal antibody (12683-1-AP)

    WB analysis of HeLa using 12683-1-AP (same clone as 12683-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12683-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using MAD2L2 Polyclonal antibody (12683-1-AP)

    WB analysis of human kidney using 12683-1-AP (same clone as 12683-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12683-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human colon tissue using MAD2L2 Polyclonal antibody (12683-1-AP)

    WB analysis of human colon using 12683-1-AP (same clone as 12683-1-PBS)

    human colon tissue were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12683-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using MAD2L2 Polyclonal antibody (12683-1-AP)

    WB analysis of HEK-293 using 12683-1-AP (same clone as 12683-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12683-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using MAD2L2 Polyclonal antibody (12683-1-AP)

    WB analysis of K-562 using 12683-1-AP (same clone as 12683-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12683-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using MAD2L2 Polyclonal antibody (12683-1-AP)

    WB analysis of fetal human brain using 12683-1-AP (same clone as 12683-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12683-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using MAD2L2 Polyclonal antibody (12683-1-AP)

    WB analysis of mouse brain using 12683-1-AP (same clone as 12683-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12683-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A375 cells using MAD2L2 Polyclonal antibody (12683-1-AP)

    WB analysis of A375 using 12683-1-AP (same clone as 12683-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12683-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using MAD2L2 Polyclonal antibody (12683-1-AP)

    IP experiment of mouse brain using 12683-1-AP (same clone as 12683-1-PBS)

    IP result of anti-MAD2L2 (IP:12683-1-AP, 3ug; Detection:12683-1-AP 1:500) with mouse brain tissue lysate 3600ug. This data was developed using the same antibody clone with 12683-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using MAD2L2 Polyclonal antibody (12683-1-AP)

    IHC staining of human ovary tumor using 12683-1-AP (same clone as 12683-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12683-1-AP (MAD2L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12683-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using MAD2L2 Polyclonal antibody (12683-1-AP)

    IHC staining of human ovary tumor using 12683-1-AP (same clone as 12683-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12683-1-AP (MAD2L2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12683-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using MAD2L2 Polyclonal antibody (12683-1-AP)

    IHC staining of human lymphoma using 12683-1-AP (same clone as 12683-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12683-1-AP (MAD2L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12683-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using MAD2L2 Polyclonal antibody (12683-1-AP)

    IHC staining of human lymphoma using 12683-1-AP (same clone as 12683-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12683-1-AP (MAD2L2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12683-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

MAD2L2 Polyclonal antibody, PBS Only

MAD2L2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 12683-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12683-1-PBS targets MAD2L2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAD2L2 fusion protein Ag3373 相同性解析による交差性が予測される生物種
Full Name MAD2 mitotic arrest deficient-like 2 (yeast)
Calculated molecular weight 211 aa, 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC015244
Gene Symbol MAD2L2
Gene ID (NCBI) 10459
RRIDAB_2139530
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UI95
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MAD family, together with BUB and Mps1,Cdc20k, play roles in the mitotic spindle checkpoint. MAD2L2 is one of the MAD family. It can mediate the second polymerase switching in translation DNA synthesis by mediating the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1. Through regulation of the JNK-mediate phosphorylation and activation of the transcriptional activator ELK1, MAD2L2 involves in cellular response to DNA damage. Also it has role in the progression of cell cycle and peithelial-mesenchymal transdifferentiation.