MAdCAM1 Monoclonal antibody

MAdCAM1 Monoclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2A12E8

Cat no : 66594-1-Ig

Synonyms

hMAdCAM 1, MACAM1, MAdCAM 1, MAdCAM1


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Tested Applications

Positive IHC detected inhuman colon tissue, human tonsillitis tissue, human appendicitis tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 2 publications below

Product Information

66594-1-Ig targets MAdCAM1 in IHC, IF-P, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MAdCAM1 fusion protein Ag16184 相同性解析による交差性が予測される生物種
Full Name mucosal vascular addressin cell adhesion molecule 1
Calculated molecular weight 406 aa, 43 kDa
GenBank accession numberBC142629
Gene symbol MADCAM1
Gene ID (NCBI) 8174
RRIDAB_2881954
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Lymphocyte recirculation through different organs is thought to be regulated by adhesion molecules ("homing receptors") recognizing tissue-specific vascular addressins on endothelium (PMID: 7511642). The mucosal vascular addressin, MAdCAM1, is an immunoglobulin superfamily adhesion receptor for lymphocytes that is expressed by mucosal venules and helps direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria (PMID: 7687523). MAdCAM1 can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes.

Protocols

Product Specific Protocols
IHC protocol for MAdCAM1 antibody 66594-1-IgDownload protocol
IF protocol for MAdCAM1 antibody 66594-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

J Crohns Colitis

Mucosal addressin cell adhesion molecule 1 expression reflects mucosal inflammation and subsequent relapse in patients with ulcerative colitis

Authors - Kazuhiko Uchiyama
humanIHC

Int J Cancer

MAdCAM-1 targeting strategy can prevent colitic cancer carcinogenesis and progression via suppression of immune cell infiltration and inflammatory signals

Authors - Naoya Ozawa