Validation Data Gallery
Tested Applications
| Positive IHC detected in | human colon tissue, human tonsillitis tissue, human appendicitis tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 2 publications below |
Product Information
66594-1-Ig targets MAdCAM1 in IHC, IF-P, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | MAdCAM1 fusion protein Ag16184 相同性解析による交差性が予測される生物種 |
| Full Name | mucosal vascular addressin cell adhesion molecule 1 |
| Calculated molecular weight | 406 aa, 43 kDa |
| GenBank accession number | BC142629 |
| Gene Symbol | MADCAM1 |
| Gene ID (NCBI) | 8174 |
| RRID | AB_2881954 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13477 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Lymphocyte recirculation through different organs is thought to be regulated by adhesion molecules ("homing receptors") recognizing tissue-specific vascular addressins on endothelium (PMID: 7511642). The mucosal vascular addressin, MAdCAM1, is an immunoglobulin superfamily adhesion receptor for lymphocytes that is expressed by mucosal venules and helps direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria (PMID: 7687523). MAdCAM1 can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MAdCAM1 antibody 66594-1-Ig | Download protocol |
| IF protocol for MAdCAM1 antibody 66594-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Crohns Colitis Mucosal addressin cell adhesion molecule 1 expression reflects mucosal inflammation and subsequent relapse in patients with ulcerative colitis | ||
Int J Cancer MAdCAM-1 targeting strategy can prevent colitic cancer carcinogenesis and progression via suppression of immune cell infiltration and inflammatory signals |