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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAdCAM1 Monoclonal antibody, PBS Only

MAdCAM1 Monoclonal Antibody for IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A12E8

Cat no : 66594-1-PBS

Synonyms

hMAdCAM 1, MACAM1, MAdCAM 1, MAdCAM1

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human colon tissue using MAdCAM1 Monoclonal antibody (66594-1-Ig)

    IHC staining of human colon using 66594-1-Ig (same clone as 66594-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66594-1-Ig (MAdCAM1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66594-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using MAdCAM1 Monoclonal antibody (66594-1-Ig)

    IHC staining of human colon using 66594-1-Ig (same clone as 66594-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66594-1-Ig (MAdCAM1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66594-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using MAdCAM1 Monoclonal antibody (66594-1-Ig)

    IHC staining of human tonsillitis using 66594-1-Ig (same clone as 66594-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66594-1-Ig (MADCAM1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66594-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using MAdCAM1 Monoclonal antibody (66594-1-Ig)

    IHC staining of human tonsillitis using 66594-1-Ig (same clone as 66594-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66594-1-Ig (MADCAM1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66594-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using MAdCAM1 Monoclonal antibody (66594-1-Ig)

    IHC staining of human appendicitis using 66594-1-Ig (same clone as 66594-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66594-1-Ig (MAdCAM1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with . This data was developed using the same antibody clone with 66594-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using MAdCAM1 Monoclonal antibody (66594-1-Ig)

    IHC staining of human appendicitis using 66594-1-Ig (same clone as 66594-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66594-1-Ig (MAdCAM1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with . This data was developed using the same antibody clone with 66594-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using MAdCAM1 Monoclonal antibody (66594-1-Ig)

    IHC staining of human small intestine using 66594-1-Ig (same clone as 66594-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66594-1-Ig (MADCAM1 antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66594-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using MAdCAM1 Monoclonal antibody (66594-1-Ig)

    IHC staining of human small intestine using 66594-1-Ig (same clone as 66594-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66594-1-Ig (MADCAM1 antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66594-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using MAdCAM1 Monoclonal antibody (66594-1-Ig)

    IHC staining of human tonsillitis using 66594-1-Ig (same clone as 66594-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66594-1-Ig (MAdCAM1 antibody) at dilution of 1:500 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66594-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using MAdCAM1 Monoclonal antibody (66594-1-Ig)

    IF Staining of human tonsillitis using 66594-1-Ig (same clone as 66594-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using MAdCAM1 antibody (66594-1-Ig, Clone: 2A12E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66594-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using MAdCAM1 Monoclonal antibody (66594-1-Ig)

    IF Staining of human tonsillitis using 66594-1-Ig (same clone as 66594-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using MAdCAM1 antibody (66594-1-Ig, Clone: 2A12E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66594-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66594-1-PBS targets MAdCAM1 in IHC, IF-P, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MAdCAM1 fusion protein Ag16184 相同性解析による交差性が予測される生物種
Full Name mucosal vascular addressin cell adhesion molecule 1
Calculated molecular weight 406 aa, 43 kDa
GenBank accession numberBC142629
Gene symbol MADCAM1
Gene ID (NCBI) 8174
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Lymphocyte recirculation through different organs is thought to be regulated by adhesion molecules ("homing receptors") recognizing tissue-specific vascular addressins on endothelium (PMID: 7511642). The mucosal vascular addressin, MAdCAM1, is an immunoglobulin superfamily adhesion receptor for lymphocytes that is expressed by mucosal venules and helps direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria (PMID: 7687523). MAdCAM1 can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes.