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  • KD/KO Validated

MAFB Polyclonal antibody

MAFB Polyclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 20189-1-AP

Synonyms

V-maf musculoaponeurotic fibrosarcoma oncogene homolog B, Transcription factor MafB, Maf-B, Maf B, KRML


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Tested Applications

Positive WB detected inRAW 264.7 cells
Positive FC (Intra) detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20189-1-AP targets MAFB in WB, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAFB fusion protein Ag14102 相同性解析による交差性が予測される生物種
Full Name v-maf musculoaponeurotic fibrosarcoma oncogene homolog B (avian)
Calculated molecular weight 323 aa, 36 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC036689
Gene symbol MAFB
Gene ID (NCBI) 9935
RRIDAB_2878654
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAFB (MAF BZIP Transcription Factor B), also known as V-maf musculoaponeurotic fibrosarcoma oncogene homolog B, is a widely expressed basic leucine zipper transcription factor belonging to the Maf family. MAFB expression is significantly elevated in response to a broad range of metabolic and immunological stimuli that promote macrophage M2 polarization, cholesterol efflux, or both, whereas it is strongly down-regulated in response to various pro-inflammatory pathogenic triggers(PMID: 28790455). MAFB is highly expressed in tumor tissues and required for proliferation and tumorigenicity of osteosarcoma cells(PMID: 32234710) and is a marker of tumor-associated macrophages in both mouse and human(PMID: 31679694).

Protocols

Product Specific Protocols
WB protocol for MAFB antibody 20189-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Microbiol

Transcription factor MAFB controls type I and II interferon response-mediated host immunity in Mycobacterium tuberculosis-infected macrophages

Authors - Haruka Hikichi
  • KD Validated
humanWB

J Exp Clin Cancer Res

Musashi2 promotes EGF-induced EMT in pancreatic cancer via ZEB1-ERK/MAPK signaling

Authors - Weiwei Sheng
ratWB

Mol Cell Endocrinol

lnc-HC ameliorates steatosis by promoting miR-130b-3p biogenesis and the assembly of an RNA-induced silencing complex

Authors - Xi Lan
  • KD Validated
mouseWB,IP

Commun Biol

MafB regulates NLRP3 inflammasome activation by sustaining p62 expression in macrophages

Authors - Huachun Cui
mouseWB

Commun Biol

The matrix vesicle cargo miR-125b accumulates in the bone matrix, inhibiting bone resorption in mice

Authors - Tomoko Minamizaki