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Validation Data Gallery

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  • Flow cytometry (FC) experiment of RAW 264.7 cells using CoraLite® Plus 488-conjugated MAFB Polyclonal anti (CL488-20189)

    FC experiment of RAW 264.7 using CL488-20189

    1x10^6 RAW 264.7 cells were intracellularly stained with 0.4 ug Coralite® Plus 488 MAFB Polyclonal Antibody (CL488-20189)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated MAFB Polyclonal antibody

MAFB Polyclonal Antibody for FC (Intra)
Cat No. CL488-20189

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Synonyms

V-maf musculoaponeurotic fibrosarcoma oncogene homolog B, Transcription factor MafB, Maf-B, Maf B, KRML

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-20189 targets MAFB in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAFB fusion protein Ag14102 相同性解析による交差性が予測される生物種
Full Name v-maf musculoaponeurotic fibrosarcoma oncogene homolog B (avian)
Calculated molecular weight 323 aa, 36 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC036689
Gene Symbol MAFB
Gene ID (NCBI) 9935
ENSEMBL Gene IDENSG00000204103
RRIDAB_3672714
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5Q3
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAFB (MAF BZIP Transcription Factor B), also known as V-maf musculoaponeurotic fibrosarcoma oncogene homolog B, is a widely expressed basic leucine zipper transcription factor belonging to the Maf family. MAFB expression is significantly elevated in response to a broad range of metabolic and immunological stimuli that promote macrophage M2 polarization, cholesterol efflux, or both, whereas it is strongly down-regulated in response to various pro-inflammatory pathogenic triggers(PMID: 28790455). MAFB is highly expressed in tumor tissues and required for proliferation and tumorigenicity of osteosarcoma cells(PMID: 32234710) and is a marker of tumor-associated macrophages in both mouse and human(PMID: 31679694).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 MAFB antibody CL488-20189Download protocol
Standard Protocols
Click here to view our Standard Protocols