CoraLite® Plus 488-conjugated MAFB Polyclonal antibody
MAFB Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-20189
Synonyms
Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-20189 targets MAFB in FC (Intra) applications and shows reactivity with human, rat, mouse samples.
| Tested Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MAFB fusion protein Ag14102 相同性解析による交差性が予測される生物種 |
| Full Name | v-maf musculoaponeurotic fibrosarcoma oncogene homolog B (avian) |
| Calculated molecular weight | 323 aa, 36 kDa |
| Observed molecular weight | 45 kDa |
| GenBank accession number | BC036689 |
| Gene symbol | MAFB |
| Gene ID (NCBI) | 9935 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MAFB (MAF BZIP Transcription Factor B), also known as V-maf musculoaponeurotic fibrosarcoma oncogene homolog B, is a widely expressed basic leucine zipper transcription factor belonging to the Maf family. MAFB expression is significantly elevated in response to a broad range of metabolic and immunological stimuli that promote macrophage M2 polarization, cholesterol efflux, or both, whereas it is strongly down-regulated in response to various pro-inflammatory pathogenic triggers(PMID: 28790455). MAFB is highly expressed in tumor tissues and required for proliferation and tumorigenicity of osteosarcoma cells(PMID: 32234710) and is a marker of tumor-associated macrophages in both mouse and human(PMID: 31679694).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 MAFB antibody CL488-20189 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
