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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAG Monoclonal antibody

MAG Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3H9G3

Cat no : 66709-1-Ig

Synonyms

GMA, MAG, myelin associated glycoprotein, S MAG, SIGLEC 4A, SIGLEC4A

Validation Data Gallery

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  • Western Blot (WB) analysis of rat brain tissue using MAG Monoclonal antibody (66709-1-Ig)

    WB analysis of rat brain using 66709-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66709-1-Ig (MAG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat cerebellum tissue using MAG Monoclonal antibody (66709-1-Ig)

    WB analysis of rat cerebellum using 66709-1-Ig

    rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 66709-1-Ig (MAG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MAG Monoclonal antibody (66709-1-Ig)

    IHC staining of mouse brain using 66709-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66709-1-Ig (MAG antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MAG Monoclonal antibody (66709-1-Ig)

    IHC staining of mouse brain using 66709-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66709-1-Ig (MAG antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inrat brain tissue, rat cerebellum tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66709-1-Ig targets MAG in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MAG fusion protein Ag6065 相同性解析による交差性が予測される生物種
Full Name myelin associated glycoprotein
Calculated molecular weight 69 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC053347
Gene symbol MAG
Gene ID (NCBI) 4099
RRIDAB_2882061
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAG (myelin associated glycoprotein) is a 100-kDa transmembrane glycoprotein that is a member of the SIGLEC family of proteins. MAG is localized in periaxonal Schwann cell and oligodendroglial membranes of myelin sheaths where it functions in glia-axon interactions (PMID: 17241126). It is a functional ligand for the Nogo-66 receptor (PMID: 12089450). MAG is a cell adhesion molecule for postnatal neural development and is thought to be involved in the process of myelination.

Protocols

Product Specific Protocols
WB protocol for MAG antibody 66709-1-IgDownload protocol
IHC protocol for MAG antibody 66709-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols