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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAG Monoclonal antibody, PBS Only

MAG Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3H9G3

Cat no : 66709-1-PBS

Synonyms

GMA, MAG, myelin associated glycoprotein, S MAG, SIGLEC 4A, SIGLEC4A

Validation Data Gallery

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  • Western Blot (WB) analysis of rat brain tissue using MAG Monoclonal antibody (66709-1-Ig)

    WB analysis of rat brain using 66709-1-Ig (same clone as 66709-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66709-1-Ig (MAG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66709-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat cerebellum tissue using MAG Monoclonal antibody (66709-1-Ig)

    WB analysis of rat cerebellum using 66709-1-Ig (same clone as 66709-1-PBS)

    rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 66709-1-Ig (MAG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66709-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MAG Monoclonal antibody (66709-1-Ig)

    IHC staining of mouse brain using 66709-1-Ig (same clone as 66709-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66709-1-Ig (MAG antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66709-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MAG Monoclonal antibody (66709-1-Ig)

    IHC staining of mouse brain using 66709-1-Ig (same clone as 66709-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66709-1-Ig (MAG antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66709-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66709-1-PBS targets MAG in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MAG fusion protein Ag6065 相同性解析による交差性が予測される生物種
Full Name myelin associated glycoprotein
Calculated molecular weight 69 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC053347
Gene symbol MAG
Gene ID (NCBI) 4099
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MAG (myelin associated glycoprotein) is a 100-kDa transmembrane glycoprotein that is a member of the SIGLEC family of proteins. MAG is localized in periaxonal Schwann cell and oligodendroglial membranes of myelin sheaths where it functions in glia-axon interactions (PMID: 17241126). It is a functional ligand for the Nogo-66 receptor (PMID: 12089450). MAG is a cell adhesion molecule for postnatal neural development and is thought to be involved in the process of myelination.