• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAGEA3 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 39.1-2500 pg/mL

Cat No : MP00467-4

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00467-4, Human MAGEA3 Recombinant Matched Antibody Pair - PBS only. 83480-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0265. 83480-3-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

    Sandwich ELISA standard curve of MP00467-4

    Sandwich ELISA standard curve of MP00467-4, Human MAGEA3 Recombinant Matched Antibody Pair - PBS only. 83480-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0265. 83480-3-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

  • Serum of fourteen individual healthy human donors was measured. The human MAGEA3 concentration of detected samples was determined to be 1,950.7 pg/mL with a range of ND - 12,398.4 pg/mL.

    Sample test of MP00467-4

    Serum of fourteen individual healthy human donors was measured. The human MAGEA3 concentration of detected samples was determined to be 1,950.7 pg/mL with a range of ND - 12,398.4 pg/mL.

  • The mean human MAGEA3 concentration was determined to be 2,719.3 pg/mL in BxPC-3 cell extract based on a 5.4 mg/mL extract load.

    Sample test of MP00467-4

    The mean human MAGEA3 concentration was determined to be 2,719.3 pg/mL in BxPC-3 cell extract based on a 5.4 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00467-4 targets MAGEA3 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 39.1-2500 pg/mL (Sandwich ELISA);

Capture antibody: MAGEA3 Recombinant antibody, PBS Only (Capture) 83480-6-PBS (240435F6). 100 μg. Concentration 1 mgl/ml.

Detection antibody: MAGEA3 Recombinant antibody, PBS Only (Capture/Detector) 83480-3-PBS (240435F8). 100 μg. Concentration 1 mgl/ml.

Alternative MAGEA3 matched antibody pairs: MP00467-1, MP00467-2, MP00467-3

Matched pair protein standard: Ag0265

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83480-6-PBS
Tested Reactivity human
Immunogen MAGEA3 fusion protein Ag0265
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name melanoma antigen family A, 3
Synonyms HIP8, CT1.3, Cancer/testis antigen 1.3, Antigen MZ2-D, Antigen MZ2 D
GenBank accession numberBC000340
Gene symbol MAGEA3
UniprotP43357
Gene ID (NCBI) 4102
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 83480-3-PBS
Tested Reactivity human
Immunogen MAGEA3 fusion protein Ag0265
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name melanoma antigen family A, 3
Synonyms HIP8, CT1.3, Cancer/testis antigen 1.3, Antigen MZ2-D, Antigen MZ2 D
GenBank accession numberBC000340
Gene symbol MAGEA3
UniprotP43357
Gene ID (NCBI) 4102
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.