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Host
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAGEA4 Recombinant antibody, PBS Only

MAGEA4 Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241558B10

Cat no : 84248-6-PBS

Synonyms

MAGE 41 antigen, MAGE 41, MAGE 4 antigen, CT1.4, Cancer/testis antigen 1.4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAGEA4 Recombinant antibody (84248-6-RR)

    WB analysis using 84248-6-RR (same clone as 84248-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84248-6-RR (MAGEA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84248-6-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using MAGEA4 Recombinant antibody (84248-6-RR)

    WB analysis of human testis using 84248-6-RR (same clone as 84248-6-PBS)

    human testis tissue was subjected to SDS PAGE followed by western blot with 84248-6-RR (MAGEA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84248-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using MAGEA4 Recombinant antibody (84248-6-RR)

    FC experiment of A431 using 84248-6-RR (same clone as 84248-6-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug MAGEA4 Recombinant antibody (84248-6-RR, Clone:241558B10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84248-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U-2 OS using MAGEA4 Recombinant antibody (84248-6-RR)

    FC experiment of U-2 OS using 84248-6-RR (same clone as 84248-6-PBS)

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug MAGEA4 Recombinant antibody (84248-6-RR, Clone:241558B10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84248-6-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 84248-6-PBS is MAGEA4 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MAGEA4 fusion protein Ag3193 相同性解析による交差性が予測される生物種
Full Name melanoma antigen family A, 4
Calculated molecular weight 317 aa, 35 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC017723
Gene symbol MAGEA4
Gene ID (NCBI) 4103
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MAGEA4 is a member of the MAGEA family, which belongs to chromosome X-clustered cancer/testis antigens. MAGEA4 is expressed in various human cancers but not in normal tissues besides the testis. High expression of MAGE-A4 in tumors was correlated with poor prognosis in many tumor types.