Validation Data Gallery
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Tested Applications
| Positive WB detected in | A431 cells, human testis tissue, U2OS cells, A375 cells |
| Positive FC (Intra) detected in | A431 cells, U-2 OS |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84248-6-RR targets MAGEA4 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | MAGEA4 fusion protein Ag3193 相同性解析による交差性が予測される生物種 |
| Full Name | melanoma antigen family A, 4 |
| Calculated molecular weight | 317 aa, 35 kDa |
| Observed molecular weight | 45 kDa |
| GenBank accession number | BC017723 |
| Gene Symbol | MAGEA4 |
| Gene ID (NCBI) | 4103 |
| RRID | AB_3671798 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P43358 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MAGEA4 is a member of the MAGEA family, which belongs to chromosome X-clustered cancer/testis antigens. MAGEA4 is expressed in various human cancers but not in normal tissues besides the testis. High expression of MAGE-A4 in tumors was correlated with poor prognosis in many tumor types.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MAGEA4 antibody 84248-6-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |