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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAGEA4 Recombinant antibody

MAGEA4 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241558B10

Cat no : 84248-6-RR

Synonyms

MAGE 41 antigen, MAGE 41, MAGE 4 antigen, CT1.4, Cancer/testis antigen 1.4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAGEA4 Recombinant antibody (84248-6-RR)

    WB analysis using 84248-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84248-6-RR (MAGEA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using MAGEA4 Recombinant antibody (84248-6-RR)

    WB analysis of human testis using 84248-6-RR

    human testis tissue was subjected to SDS PAGE followed by western blot with 84248-6-RR (MAGEA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A431 cells using MAGEA4 Recombinant antibody (84248-6-RR)

    FC experiment of A431 using 84248-6-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug MAGEA4 Recombinant antibody (84248-6-RR, Clone:241558B10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Flow cytometry (FC) experiment of U-2 OS using MAGEA4 Recombinant antibody (84248-6-RR)

    FC experiment of U-2 OS using 84248-6-RR

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug MAGEA4 Recombinant antibody (84248-6-RR, Clone:241558B10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Tested Applications

Positive WB detected inA431 cells, human testis tissue, U2OS cells, A375 cells
Positive FC (Intra) detected inA431 cells, U-2 OS

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84248-6-RR is MAGEA4 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MAGEA4 fusion protein Ag3193 相同性解析による交差性が予測される生物種
Full Name melanoma antigen family A, 4
Calculated molecular weight 317 aa, 35 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC017723
Gene symbol MAGEA4
Gene ID (NCBI) 4103
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAGEA4 is a member of the MAGEA family, which belongs to chromosome X-clustered cancer/testis antigens. MAGEA4 is expressed in various human cancers but not in normal tissues besides the testis. High expression of MAGE-A4 in tumors was correlated with poor prognosis in many tumor types.

Protocols

Product Specific Protocols
WB protocol for MAGEA4 antibody 84248-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols